Results 1 - 20 of 95 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26453 | 3' | -64.4 | NC_005357.1 | + | 41603 | 0.65 | 0.241453 |
Target: 5'- aGGGCCGCCGcguuguuccagaagUUGaCCGCGCUuucGUAGCc -3' miRNA: 3'- -UCCGGCGGC--------------AGC-GGCGCGAcc-CGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 36760 | 0.66 | 0.237238 |
Target: 5'- -uGUCGCacaGaUCGCCcagcGCGCcGGGCAGCg -3' miRNA: 3'- ucCGGCGg--C-AGCGG----CGCGaCCCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 22797 | 0.66 | 0.23664 |
Target: 5'- cGGGCCggucagcaugguGCCGgacuggcccgacuUCGCCGCGCUGc-CGACa -3' miRNA: 3'- -UCCGG------------CGGC-------------AGCGGCGCGACccGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 40402 | 0.66 | 0.231322 |
Target: 5'- gAGGCgGCCGguaUGCCgGUGCU-GGUGGCg -3' miRNA: 3'- -UCCGgCGGCa--GCGG-CGCGAcCCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 748 | 0.66 | 0.231322 |
Target: 5'- cGGCCcgGCCGgCGCCGC-CguaGGCGGCc -3' miRNA: 3'- uCCGG--CGGCaGCGGCGcGac-CCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 3543 | 0.66 | 0.231322 |
Target: 5'- cGGGCCGUCGuucUCGuUCGCGUucuucgGGGCGcGCg -3' miRNA: 3'- -UCCGGCGGC---AGC-GGCGCGa-----CCCGU-UG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 16937 | 0.66 | 0.230738 |
Target: 5'- aGGGCacggCGCCGUCcagcagcguGCCGCGUUGGuauggaaGCGAa -3' miRNA: 3'- -UCCG----GCGGCAG---------CGGCGCGACC-------CGUUg -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 12092 | 0.66 | 0.230738 |
Target: 5'- uGGGCaagaccuCGCCGgccacgGCCGCGCUGcuGGcCGACg -3' miRNA: 3'- -UCCG-------GCGGCag----CGGCGCGAC--CC-GUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 8748 | 0.66 | 0.230738 |
Target: 5'- aGGGCgCGCgCGUauucacgUGCCGCuucGGGCAGCa -3' miRNA: 3'- -UCCG-GCG-GCA-------GCGGCGcgaCCCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 19980 | 0.66 | 0.227833 |
Target: 5'- cAGGCCGCCGauguccgcguagaacUCGCCaacuugcucggcaugGCGCgccugcaucacaGGCAGCa -3' miRNA: 3'- -UCCGGCGGC---------------AGCGG---------------CGCGac----------CCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 8977 | 0.66 | 0.225532 |
Target: 5'- cGGUaaCGCCucgauuuccUCGCCGCGCggGGGCGc- -3' miRNA: 3'- uCCG--GCGGc--------AGCGGCGCGa-CCCGUug -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 21338 | 0.66 | 0.219865 |
Target: 5'- uGGGCCGacaCuuaCGCCGacauGCUGGGCAu- -3' miRNA: 3'- -UCCGGCg--Gca-GCGGCg---CGACCCGUug -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 38387 | 0.66 | 0.219865 |
Target: 5'- uGGCCuGCCGcUCGCCGCGgccGGCu-- -3' miRNA: 3'- uCCGG-CGGC-AGCGGCGCgacCCGuug -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 15846 | 0.66 | 0.219865 |
Target: 5'- -cGCCGuCCGU-GUCGUGCUGGuGCcACg -3' miRNA: 3'- ucCGGC-GGCAgCGGCGCGACC-CGuUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 25829 | 0.66 | 0.219305 |
Target: 5'- cGGGCCGCCGUUGgCGCcggucaguuccauGCUGGu---- -3' miRNA: 3'- -UCCGGCGGCAGCgGCG-------------CGACCcguug -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 37185 | 0.66 | 0.21432 |
Target: 5'- cAGGUCgagGCCGUagGCCGCGagcacgUGGcGCAGCa -3' miRNA: 3'- -UCCGG---CGGCAg-CGGCGCg-----ACC-CGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 18055 | 0.66 | 0.21432 |
Target: 5'- gGGGCCuuaCCGgCGCggggugCGUGCUGGGCcGCc -3' miRNA: 3'- -UCCGGc--GGCaGCG------GCGCGACCCGuUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 16793 | 0.66 | 0.21432 |
Target: 5'- uGGUCGCCaGUUGCCaguuggucGCGCccaGGCGGCg -3' miRNA: 3'- uCCGGCGG-CAGCGG--------CGCGac-CCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 5632 | 0.66 | 0.208895 |
Target: 5'- cGGCCagcguuggcGCCGUCGauaCCGUGgC-GGGCGACc -3' miRNA: 3'- uCCGG---------CGGCAGC---GGCGC-GaCCCGUUG- -5' |
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26453 | 3' | -64.4 | NC_005357.1 | + | 29540 | 0.66 | 0.208895 |
Target: 5'- uGGCCGCagaGgacacccaCGCCGUGCUcgGGGUAucGCg -3' miRNA: 3'- uCCGGCGg--Ca-------GCGGCGCGA--CCCGU--UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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