Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26454 | 5' | -50.4 | NC_005357.1 | + | 7253 | 0.66 | 0.882742 |
Target: 5'- gCGGGCcGAUGUCGccggccuuGUGCAGCuucucggcgguGGUCGGa -3' miRNA: 3'- -GUUCGcUUGUAGU--------UACGUCG-----------CCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 40581 | 0.66 | 0.900904 |
Target: 5'- -cGGCGAGgAUgAAucucUGCAGCGGuuucucgccucguggUCGGg -3' miRNA: 3'- guUCGCUUgUAgUU----ACGUCGCC---------------AGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 39236 | 0.66 | 0.897994 |
Target: 5'- uCAGGUGAGCAagCuguacGCAGCGGacgcCGGg -3' miRNA: 3'- -GUUCGCUUGUa-Guua--CGUCGCCa---GCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 35250 | 0.66 | 0.905179 |
Target: 5'- -cGGCu-GCGUCAggGCgucAGCGcGUCGGg -3' miRNA: 3'- guUCGcuUGUAGUuaCG---UCGC-CAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 25906 | 0.66 | 0.905179 |
Target: 5'- uCAGGCGggUuUCGAcuUGCAGCuugCGGu -3' miRNA: 3'- -GUUCGCuuGuAGUU--ACGUCGccaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 24332 | 0.66 | 0.905179 |
Target: 5'- uCAGGCG-GCAgagCGAUGCccguGUuguuGGUCGGg -3' miRNA: 3'- -GUUCGCuUGUa--GUUACGu---CG----CCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 7327 | 0.66 | 0.897994 |
Target: 5'- --uGCGAACG-CA--GCAGCcGGUUGGc -3' miRNA: 3'- guuCGCUUGUaGUuaCGUCG-CCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 23416 | 0.66 | 0.897994 |
Target: 5'- uGAGCGcGCcgGUCAGUGCGacGCcGGcCGGg -3' miRNA: 3'- gUUCGCuUG--UAGUUACGU--CG-CCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 17758 | 0.66 | 0.874685 |
Target: 5'- cCAGGUGuGCAguUCGA-GCAGCGaGUCGc -3' miRNA: 3'- -GUUCGCuUGU--AGUUaCGUCGC-CAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 10081 | 0.66 | 0.890513 |
Target: 5'- -cGGCGAccGCAgCGAccuggGCGGCGGuugUCGGg -3' miRNA: 3'- guUCGCU--UGUaGUUa----CGUCGCC---AGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 3799 | 0.66 | 0.890513 |
Target: 5'- -cGGuCGGGCAgc-AUGaccaGGCGGUCGGg -3' miRNA: 3'- guUC-GCUUGUaguUACg---UCGCCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 9516 | 0.67 | 0.848879 |
Target: 5'- -uGGCGAACAguggCAucAUGCuGCGGcccuguUCGGc -3' miRNA: 3'- guUCGCUUGUa---GU--UACGuCGCC------AGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 4523 | 0.67 | 0.848879 |
Target: 5'- --cGUGAACGUgGcGUGCugGGCGGUCGu -3' miRNA: 3'- guuCGCUUGUAgU-UACG--UCGCCAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 41960 | 0.67 | 0.839761 |
Target: 5'- -uGGCGAuuGUCGAaGCGGUGGUgcgCGGc -3' miRNA: 3'- guUCGCUugUAGUUaCGUCGCCA---GCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 40155 | 0.67 | 0.830401 |
Target: 5'- -cAGCGAACccaucGUCAacGUGguGCGcGUCGa -3' miRNA: 3'- guUCGCUUG-----UAGU--UACguCGC-CAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 14574 | 0.67 | 0.848879 |
Target: 5'- --cGCGGACAcguUCuugaGCAGCGGcgCGGc -3' miRNA: 3'- guuCGCUUGU---AGuua-CGUCGCCa-GCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 26031 | 0.68 | 0.82081 |
Target: 5'- -cGGCGAugGCAUCAAaGCccgcGCGG-CGGg -3' miRNA: 3'- guUCGCU--UGUAGUUaCGu---CGCCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 14752 | 0.68 | 0.80098 |
Target: 5'- --cGCGcAGCAUUGGcgcGCAGCGuGUCGGu -3' miRNA: 3'- guuCGC-UUGUAGUUa--CGUCGC-CAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 10308 | 0.68 | 0.790766 |
Target: 5'- --cGgGGGCGUCGAggucgGCAGCGGcCaGGg -3' miRNA: 3'- guuCgCUUGUAGUUa----CGUCGCCaG-CC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 21521 | 0.68 | 0.810999 |
Target: 5'- aAGGCuGGGCAUCGAcacguUGcCGGCaGUCGGu -3' miRNA: 3'- gUUCG-CUUGUAGUU-----AC-GUCGcCAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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