Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26454 | 5' | -50.4 | NC_005357.1 | + | 37339 | 1.1 | 0.001908 |
Target: 5'- cCAAGCGAACAUCAAUGCAGCGGUCGGc -3' miRNA: 3'- -GUUCGCUUGUAGUUACGUCGCCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 37399 | 0.83 | 0.145278 |
Target: 5'- cCAGGCGAAUAUCAAcGCAGUaGUCGGu -3' miRNA: 3'- -GUUCGCUUGUAGUUaCGUCGcCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 37365 | 0.82 | 0.163103 |
Target: 5'- gCAAuGcCGAcaACAUCAAUGCAGCGGUCGc -3' miRNA: 3'- -GUU-C-GCU--UGUAGUUACGUCGCCAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 39657 | 0.78 | 0.284145 |
Target: 5'- --uGCGcAACGUCGAggugucgGCAGUGGUCGGc -3' miRNA: 3'- guuCGC-UUGUAGUUa------CGUCGCCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 21806 | 0.76 | 0.349531 |
Target: 5'- -uGGCGGACAccggCAGuUGCAGCGG-CGGg -3' miRNA: 3'- guUCGCUUGUa---GUU-ACGUCGCCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 24699 | 0.76 | 0.376668 |
Target: 5'- cCAGGCuGucCAUCGGUGcCAGuCGGUCGGg -3' miRNA: 3'- -GUUCG-CuuGUAGUUAC-GUC-GCCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 9323 | 0.73 | 0.509254 |
Target: 5'- -cGGCGcACGUCGGcGCGGCGGgCGGc -3' miRNA: 3'- guUCGCuUGUAGUUaCGUCGCCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 36840 | 0.73 | 0.520304 |
Target: 5'- gAGGCGGACAUCGAcaCGGUGG-CGGc -3' miRNA: 3'- gUUCGCUUGUAGUUacGUCGCCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 37162 | 0.72 | 0.576791 |
Target: 5'- --cGCGAACAUCAAcaCGGUGGcCGGu -3' miRNA: 3'- guuCGCUUGUAGUUacGUCGCCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 34371 | 0.7 | 0.669212 |
Target: 5'- -cGGUGAACugaugGUCGA-GCAGCGGUUGa -3' miRNA: 3'- guUCGCUUG-----UAGUUaCGUCGCCAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 39525 | 0.7 | 0.703557 |
Target: 5'- --cGCGAACAUCGggGCGcGCGG-CGu -3' miRNA: 3'- guuCGCUUGUAGUuaCGU-CGCCaGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 10308 | 0.68 | 0.790766 |
Target: 5'- --cGgGGGCGUCGAggucgGCAGCGGcCaGGg -3' miRNA: 3'- guuCgCUUGUAGUUa----CGUCGCCaG-CC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 14752 | 0.68 | 0.80098 |
Target: 5'- --cGCGcAGCAUUGGcgcGCAGCGuGUCGGu -3' miRNA: 3'- guuCGC-UUGUAGUUa--CGUCGC-CAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 21521 | 0.68 | 0.810999 |
Target: 5'- aAGGCuGGGCAUCGAcacguUGcCGGCaGUCGGu -3' miRNA: 3'- gUUCG-CUUGUAGUU-----AC-GUCGcCAGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 26031 | 0.68 | 0.82081 |
Target: 5'- -cGGCGAugGCAUCAAaGCccgcGCGG-CGGg -3' miRNA: 3'- guUCGCU--UGUAGUUaCGu---CGCCaGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 40155 | 0.67 | 0.830401 |
Target: 5'- -cAGCGAACccaucGUCAacGUGguGCGcGUCGa -3' miRNA: 3'- guUCGCUUG-----UAGU--UACguCGC-CAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 41960 | 0.67 | 0.839761 |
Target: 5'- -uGGCGAuuGUCGAaGCGGUGGUgcgCGGc -3' miRNA: 3'- guUCGCUugUAGUUaCGUCGCCA---GCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 9516 | 0.67 | 0.848879 |
Target: 5'- -uGGCGAACAguggCAucAUGCuGCGGcccuguUCGGc -3' miRNA: 3'- guUCGCUUGUa---GU--UACGuCGCC------AGCC- -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 4523 | 0.67 | 0.848879 |
Target: 5'- --cGUGAACGUgGcGUGCugGGCGGUCGu -3' miRNA: 3'- guuCGCUUGUAgU-UACG--UCGCCAGCc -5' |
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26454 | 5' | -50.4 | NC_005357.1 | + | 14574 | 0.67 | 0.848879 |
Target: 5'- --cGCGGACAcguUCuugaGCAGCGGcgCGGc -3' miRNA: 3'- guuCGCUUGU---AGuua-CGUCGCCa-GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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