Results 21 - 34 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26456 | 3' | -57.1 | NC_005357.1 | + | 3869 | 0.67 | 0.466771 |
Target: 5'- aGGCGCCGUCgcuguaGGcCGCGCugCcGUCGc -3' miRNA: 3'- cUCGUGGCAG------UC-GUGCGugGaCAGCu -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 27474 | 0.67 | 0.466771 |
Target: 5'- -cGCGCCG-C-GCACGCACCacgCGAg -3' miRNA: 3'- cuCGUGGCaGuCGUGCGUGGacaGCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 33851 | 0.67 | 0.476977 |
Target: 5'- -cGCcgugACCGUCGuGU-CGCGCCUGUCGc -3' miRNA: 3'- cuCG----UGGCAGU-CGuGCGUGGACAGCu -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 14641 | 0.67 | 0.476977 |
Target: 5'- cGGCugCG-CGGCGCGCGCUucguguacgUGUcCGAg -3' miRNA: 3'- cUCGugGCaGUCGUGCGUGG---------ACA-GCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 413 | 0.67 | 0.487292 |
Target: 5'- cAGCACggcggugauCGUCGGC-CGUACCuUGUCGu -3' miRNA: 3'- cUCGUG---------GCAGUCGuGCGUGG-ACAGCu -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 6703 | 0.66 | 0.497711 |
Target: 5'- aGGCACaccuuGUCcugGGCGCGCAUCUGcUUGAg -3' miRNA: 3'- cUCGUGg----CAG---UCGUGCGUGGAC-AGCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 19593 | 0.66 | 0.529536 |
Target: 5'- -cGCGCUGUCGGCugucggcaGCGCGgCgaaGUCGGg -3' miRNA: 3'- cuCGUGGCAGUCG--------UGCGUgGa--CAGCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 28944 | 0.66 | 0.529536 |
Target: 5'- aGGCGCgGUCGGCAuCGC-CCagGUCa- -3' miRNA: 3'- cUCGUGgCAGUCGU-GCGuGGa-CAGcu -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 26320 | 0.66 | 0.551163 |
Target: 5'- aGGCACgGUCuuGaCGCGCACCg--CGAa -3' miRNA: 3'- cUCGUGgCAGu-C-GUGCGUGGacaGCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 22070 | 0.66 | 0.551163 |
Target: 5'- cGGGuCGCgGUCGGCGCGCGgC--UCGAu -3' miRNA: 3'- -CUC-GUGgCAGUCGUGCGUgGacAGCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 13289 | 0.66 | 0.540313 |
Target: 5'- cAGCGCCGauugCAGCGCGCcgggGCCggcGUUGu -3' miRNA: 3'- cUCGUGGCa---GUCGUGCG----UGGa--CAGCu -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 38726 | 0.66 | 0.518839 |
Target: 5'- -uGCGCCGcauggggCAGCGCGuCGCCgugGUgGAu -3' miRNA: 3'- cuCGUGGCa------GUCGUGC-GUGGa--CAgCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 9186 | 0.66 | 0.508229 |
Target: 5'- uGGCGCCGUCG--ACGUugGCCUGcUCGGc -3' miRNA: 3'- cUCGUGGCAGUcgUGCG--UGGAC-AGCU- -5' |
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26456 | 3' | -57.1 | NC_005357.1 | + | 25043 | 0.66 | 0.529536 |
Target: 5'- aAGCGgugCGUCGGCGCGaugcaggcaUACCUGUCGc -3' miRNA: 3'- cUCGUg--GCAGUCGUGC---------GUGGACAGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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