Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26461 | 5' | -59.3 | NC_005357.1 | + | 29869 | 0.66 | 0.437499 |
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Target: 5'- gCGcAGCGCgaggcCGGCaagaaauucaccgaCgCCGAGGUCGAGCa -3' miRNA: 3'- -GC-UCGUGau---GCCG--------------G-GGCUCCGGUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 33784 | 0.66 | 0.440384 |
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Target: 5'- gCGAGUACggcGCGGCCuggggcguuuCCGAagggcguaccGGCCAcGCc -3' miRNA: 3'- -GCUCGUGa--UGCCGG----------GGCU----------CCGGUuCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 15584 | 0.66 | 0.440384 |
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Target: 5'- aCGAcGCGCUGCGGaugcaCCgCGccaucGGCCAGGa -3' miRNA: 3'- -GCU-CGUGAUGCCg----GG-GCu----CCGGUUCg -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 16574 | 0.66 | 0.440384 |
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Target: 5'- uGuAGCGCgACGcGCCggaCGAGGCCGAccacGCg -3' miRNA: 3'- gC-UCGUGaUGC-CGGg--GCUCCGGUU----CG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 33185 | 0.66 | 0.440384 |
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Target: 5'- uGGGCGCccucggcACGGCCaCCGcuGGCUAucgcGGCa -3' miRNA: 3'- gCUCGUGa------UGCCGG-GGCu-CCGGU----UCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 27054 | 0.66 | 0.440384 |
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Target: 5'- --cGCACgcggACGcGCUcaCCGAGGUgAAGCa -3' miRNA: 3'- gcuCGUGa---UGC-CGG--GGCUCCGgUUCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 11325 | 0.66 | 0.45008 |
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Target: 5'- uCGGGCAgaACGGCCCauucguucAGGUCuuGCa -3' miRNA: 3'- -GCUCGUgaUGCCGGGgc------UCCGGuuCG- -5' |
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| 26461 | 5' | -59.3 | NC_005357.1 | + | 36859 | 0.66 | 0.45008 |
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Target: 5'- gGAGUAUgcCGGCCUCGAcaucgaaGCCAuGCg -3' miRNA: 3'- gCUCGUGauGCCGGGGCUc------CGGUuCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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