Results 1 - 20 of 54 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26539 | 3' | -59.2 | NC_005357.1 | + | 14161 | 0.66 | 0.409833 |
Target: 5'- cAGCgcGCGGUCAUggugcgcaacaUGGUgaGCCUGGCg -3' miRNA: 3'- -UCGaaCGUCGGUGa----------ACCGg-CGGACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 2830 | 0.66 | 0.407015 |
Target: 5'- uGCUcGCGGCCACgcgcagcgGcGCCagcaccagcgagggGCCUGGUu -3' miRNA: 3'- uCGAaCGUCGGUGaa------C-CGG--------------CGGACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 18065 | 0.66 | 0.391277 |
Target: 5'- cGGCgcggGguGCgUGCUgGGCCGCCgaagccGGCg -3' miRNA: 3'- -UCGaa--CguCG-GUGAaCCGGCGGa-----CCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 36041 | 0.66 | 0.391277 |
Target: 5'- cGaggGC-GCCGCggcGGCCGaCCUGGUg -3' miRNA: 3'- uCgaaCGuCGGUGaa-CCGGC-GGACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 39571 | 0.66 | 0.382207 |
Target: 5'- uGGCUcUGCcaacGCUacgGCUUGGCgGgCUGGCc -3' miRNA: 3'- -UCGA-ACGu---CGG---UGAACCGgCgGACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 9210 | 0.66 | 0.373278 |
Target: 5'- cGGCggcGCGGCgCACguucugcGGCgCGCCcGGCg -3' miRNA: 3'- -UCGaa-CGUCG-GUGaa-----CCG-GCGGaCCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 12870 | 0.66 | 0.373278 |
Target: 5'- cAGCUUGUuguagAGCCACaggcucgUGGUgucguCGCCUuuGGCg -3' miRNA: 3'- -UCGAACG-----UCGGUGa------ACCG-----GCGGA--CCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 34189 | 0.66 | 0.364491 |
Target: 5'- cGCUUGaagcgcaAGCUGCUgacGGCCGCCgaccaGCg -3' miRNA: 3'- uCGAACg------UCGGUGAa--CCGGCGGac---CG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 34021 | 0.66 | 0.364491 |
Target: 5'- aAGCgcaCGGCC-C-UGGCCGaCUGGCu -3' miRNA: 3'- -UCGaacGUCGGuGaACCGGCgGACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 27435 | 0.66 | 0.364491 |
Target: 5'- cGCgcccgGCGGCCGCa-GGCCcugGCgCUGGUa -3' miRNA: 3'- uCGaa---CGUCGGUGaaCCGG---CG-GACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 1962 | 0.66 | 0.361883 |
Target: 5'- gAGCgcGCGGCCcugggggaaggcacGCUguaGGCCGCgaGGUc -3' miRNA: 3'- -UCGaaCGUCGG--------------UGAa--CCGGCGgaCCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 18774 | 0.67 | 0.355849 |
Target: 5'- cGCcuggUGCAGUUGCUgcccGCCGCC-GGCu -3' miRNA: 3'- uCGa---ACGUCGGUGAac--CGGCGGaCCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 9989 | 0.67 | 0.355849 |
Target: 5'- gGGCguagggGCAGUCGCUgccGUCGUCUuGGCa -3' miRNA: 3'- -UCGaa----CGUCGGUGAac-CGGCGGA-CCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 41452 | 0.67 | 0.354993 |
Target: 5'- cGGCcagGCGGCCAagUGGCUgcaagcuGCCgugGGCa -3' miRNA: 3'- -UCGaa-CGUCGGUgaACCGG-------CGGa--CCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 9818 | 0.67 | 0.347352 |
Target: 5'- cGGCgucGCGGCCcuugACgcgGGCCaGCUUGGUc -3' miRNA: 3'- -UCGaa-CGUCGG----UGaa-CCGG-CGGACCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 32642 | 0.67 | 0.339001 |
Target: 5'- cGGCgUGC-GCgACcUGGCCGCgUGGa -3' miRNA: 3'- -UCGaACGuCGgUGaACCGGCGgACCg -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 7710 | 0.67 | 0.339001 |
Target: 5'- cGGCUugccgaugaacUGCAGCgCACgcucGGCgGCC-GGCu -3' miRNA: 3'- -UCGA-----------ACGUCG-GUGaa--CCGgCGGaCCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 16367 | 0.67 | 0.339001 |
Target: 5'- uGUUcUGCGGCUugUUGGUagUGCC-GGCa -3' miRNA: 3'- uCGA-ACGUCGGugAACCG--GCGGaCCG- -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 33761 | 0.67 | 0.331611 |
Target: 5'- cGGCgcaGCAGCCGCUgcacacagcgaguacGGCgCGgCCUGGg -3' miRNA: 3'- -UCGaa-CGUCGGUGAa--------------CCG-GC-GGACCg -5' |
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26539 | 3' | -59.2 | NC_005357.1 | + | 1754 | 0.67 | 0.330797 |
Target: 5'- ----gGCGGUCAUggccGGCCugcGCCUGGCg -3' miRNA: 3'- ucgaaCGUCGGUGaa--CCGG---CGGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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