Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26542 | 3' | -54.8 | NC_005357.1 | + | 16685 | 0.7 | 0.408787 |
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Target: 5'- --cAGCGCGCCCAGcG-UGCUGCc-- -3' miRNA: 3'- acaUCGCGCGGGUUaCuGCGACGaag -5' |
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| 26542 | 3' | -54.8 | NC_005357.1 | + | 18667 | 0.7 | 0.371319 |
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Target: 5'- --cGGCGCaucgGCCCGguggcgccggcAUGGCGCUGCUg- -3' miRNA: 3'- acaUCGCG----CGGGU-----------UACUGCGACGAag -5' |
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| 26542 | 3' | -54.8 | NC_005357.1 | + | 16382 | 0.71 | 0.336262 |
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Target: 5'- aUGgAGCGCGCCCGcc-GCGCggGCUUUg -3' miRNA: 3'- -ACaUCGCGCGGGUuacUGCGa-CGAAG- -5' |
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| 26542 | 3' | -54.8 | NC_005357.1 | + | 8065 | 0.71 | 0.336262 |
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Target: 5'- ---cGCGCGCCCAGgu-CGCUGCc-- -3' miRNA: 3'- acauCGCGCGGGUUacuGCGACGaag -5' |
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| 26542 | 3' | -54.8 | NC_005357.1 | + | 28017 | 0.75 | 0.197578 |
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Target: 5'- --aGGCGCGCCUGGcGACGCccaGCUUCg -3' miRNA: 3'- acaUCGCGCGGGUUaCUGCGa--CGAAG- -5' |
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| 26542 | 3' | -54.8 | NC_005357.1 | + | 25135 | 0.76 | 0.171747 |
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Target: 5'- aUGgauGCGCGCCCGcgG-CGCcgGCUUCg -3' miRNA: 3'- -ACau-CGCGCGGGUuaCuGCGa-CGAAG- -5' |
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| 26542 | 3' | -54.8 | NC_005357.1 | + | 2100 | 1.11 | 0.000482 |
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Target: 5'- aUGUAGCGCGCCCAAUGACGCUGCUUCa -3' miRNA: 3'- -ACAUCGCGCGGGUUACUGCGACGAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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