Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26542 | 3' | -54.8 | NC_005357.1 | + | 25092 | 0.68 | 0.521705 |
Target: 5'- --cAGCGCGCCCGGcGcCGUgggauacagcgggUGCUUCu -3' miRNA: 3'- acaUCGCGCGGGUUaCuGCG-------------ACGAAG- -5' |
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26542 | 3' | -54.8 | NC_005357.1 | + | 25135 | 0.76 | 0.171747 |
Target: 5'- aUGgauGCGCGCCCGcgG-CGCcgGCUUCg -3' miRNA: 3'- -ACau-CGCGCGGGUuaCuGCGa-CGAAG- -5' |
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26542 | 3' | -54.8 | NC_005357.1 | + | 27293 | 0.68 | 0.522802 |
Target: 5'- ---cGCGCGCCU--UGGCGaagGCUUCc -3' miRNA: 3'- acauCGCGCGGGuuACUGCga-CGAAG- -5' |
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26542 | 3' | -54.8 | NC_005357.1 | + | 28017 | 0.75 | 0.197578 |
Target: 5'- --aGGCGCGCCUGGcGACGCccaGCUUCg -3' miRNA: 3'- acaUCGCGCGGGUUaCUGCGa--CGAAG- -5' |
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26542 | 3' | -54.8 | NC_005357.1 | + | 32688 | 0.68 | 0.511874 |
Target: 5'- --aAGCGCGCCCAccacGAgcaguucgccCGCgGCUUCg -3' miRNA: 3'- acaUCGCGCGGGUua--CU----------GCGaCGAAG- -5' |
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26542 | 3' | -54.8 | NC_005357.1 | + | 34863 | 0.67 | 0.533818 |
Target: 5'- cGUGGaCGUGUCCAagccgGUGGCGCcGCggaUCg -3' miRNA: 3'- aCAUC-GCGCGGGU-----UACUGCGaCGa--AG- -5' |
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26542 | 3' | -54.8 | NC_005357.1 | + | 42139 | 0.68 | 0.501041 |
Target: 5'- --gAGCGCGCCUuccGGCGCaGCUa- -3' miRNA: 3'- acaUCGCGCGGGuuaCUGCGaCGAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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