Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26603 | 3' | -60.8 | NC_005357.1 | + | 33680 | 0.7 | 0.222959 |
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Target: 5'- gCGGcacGUGAauacgcgcgcGCCCUGGUGUCCCGGCu -3' miRNA: 3'- gGCCuc-UAUU----------CGGGGCCGCGGGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 34744 | 0.66 | 0.362902 |
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Target: 5'- gCCGccGAGGUcAAGCCCgacgaccUGGC-CCCCGGUg -3' miRNA: 3'- -GGC--CUCUA-UUCGGG-------GCCGcGGGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 35351 | 0.69 | 0.228741 |
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Target: 5'- gCCGuGGGUAGGCCgCGGCGUcaugaaguaCCUGGCc -3' miRNA: 3'- -GGCcUCUAUUCGGgGCCGCG---------GGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 36057 | 0.67 | 0.347223 |
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Target: 5'- gCCGaccuGGUGuaaCCCaGCGCCCCGGCc -3' miRNA: 3'- -GGCcu--CUAUucgGGGcCGCGGGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 36136 | 1.12 | 0.00014 |
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Target: 5'- aCCGGAGAUAAGCCCCGGCGCCCCAGCc -3' miRNA: 3'- -GGCCUCUAUUCGGGGCCGCGGGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 36655 | 0.72 | 0.14629 |
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Target: 5'- gCGGGacacGAUGgucaaGGCCCUGGCGCacucgcugCCCGGCg -3' miRNA: 3'- gGCCU----CUAU-----UCGGGGCCGCG--------GGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 41728 | 0.66 | 0.389521 |
|
Target: 5'- cCUGGGcGGUAucuugggcGGCCcggCCGGCGCCgCCguaGGCg -3' miRNA: 3'- -GGCCU-CUAU--------UCGG---GGCCGCGG-GG---UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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