Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26603 | 3' | -60.8 | NC_005357.1 | + | 19032 | 0.66 | 0.363741 |
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Target: 5'- gCGGu----AGCCCUGGUagcgGCCgCCGGCg -3' miRNA: 3'- gGCCucuauUCGGGGCCG----CGG-GGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 13068 | 0.66 | 0.367109 |
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Target: 5'- gCUGGGGcaAGGCCaCCGGCGCgUgguucgaggacuuggCCGGCg -3' miRNA: 3'- -GGCCUCuaUUCGG-GGCCGCG-G---------------GGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 12810 | 0.66 | 0.380795 |
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Target: 5'- cCCGGccacAAGCgCCCGGCGCUggacaaCUGGCa -3' miRNA: 3'- -GGCCucuaUUCG-GGGCCGCGG------GGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 31208 | 0.66 | 0.386015 |
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Target: 5'- aCGGucGAgggcgaaagcaugAAGaaCCCCGGCGCgcggCCCAGCu -3' miRNA: 3'- gGCCu-CUa------------UUC--GGGGCCGCG----GGGUCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 15939 | 0.66 | 0.389521 |
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Target: 5'- ----cGAUAGGCUgCGGCGCCUUcGCg -3' miRNA: 3'- ggccuCUAUUCGGgGCCGCGGGGuCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 41728 | 0.66 | 0.389521 |
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Target: 5'- cCUGGGcGGUAucuugggcGGCCcggCCGGCGCCgCCguaGGCg -3' miRNA: 3'- -GGCCU-CUAU--------UCGG---GGCCGCGG-GG---UCG- -5' |
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| 26603 | 3' | -60.8 | NC_005357.1 | + | 13343 | 0.66 | 0.398376 |
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Target: 5'- aCGGGccUGA-CCUCGGCGCCCgAGg -3' miRNA: 3'- gGCCUcuAUUcGGGGCCGCGGGgUCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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