Results 1 - 20 of 74 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26623 | 3' | -52.4 | NC_005357.1 | + | 42091 | 0.66 | 0.773743 |
Target: 5'- gGCGagcACGUGUGGCAcgACCcccggUACGgCACg -3' miRNA: 3'- aUGU---UGUACGCCGUa-UGG-----AUGCgGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 2135 | 0.66 | 0.773743 |
Target: 5'- gUGCAGCAgcCGGUcaACC-GCGCCGCc -3' miRNA: 3'- -AUGUUGUacGCCGuaUGGaUGCGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 9232 | 0.66 | 0.773743 |
Target: 5'- cGCGAUAgccaGCGGUggccGUGCCgaggGCGcCCACu -3' miRNA: 3'- aUGUUGUa---CGCCG----UAUGGa---UGC-GGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 25303 | 0.66 | 0.773743 |
Target: 5'- ----uCGUGCGGCcgGCCc-UGCCACc -3' miRNA: 3'- auguuGUACGCCGuaUGGauGCGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 33814 | 0.66 | 0.773743 |
Target: 5'- aAgGGCGUacCGGCcacGCCUACGCCAUc -3' miRNA: 3'- aUgUUGUAc-GCCGua-UGGAUGCGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 14767 | 0.66 | 0.773743 |
Target: 5'- cGCAGCGUGuCGGUGUcgAUCUGCuUCACg -3' miRNA: 3'- aUGUUGUAC-GCCGUA--UGGAUGcGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 13622 | 0.66 | 0.762995 |
Target: 5'- gACAGCAUcgguaGCGGCAgcaaguCCgGCGCgGCc -3' miRNA: 3'- aUGUUGUA-----CGCCGUau----GGaUGCGgUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 1080 | 0.66 | 0.762995 |
Target: 5'- aAC-GCGcGCGGUAgauUGCCUugGCCGu -3' miRNA: 3'- aUGuUGUaCGCCGU---AUGGAugCGGUg -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 4047 | 0.66 | 0.752096 |
Target: 5'- cGCGGCGaGCGGCAgGCCaGCgGCC-Ca -3' miRNA: 3'- aUGUUGUaCGCCGUaUGGaUG-CGGuG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 8208 | 0.66 | 0.752096 |
Target: 5'- aGCGACAUgGCGGCcaguuCgUGCGCgGCc -3' miRNA: 3'- aUGUUGUA-CGCCGuau--GgAUGCGgUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 22311 | 0.66 | 0.752096 |
Target: 5'- gGCAGCAcgaugcUGCuGGcCGUGCCgaacGCGCCGa -3' miRNA: 3'- aUGUUGU------ACG-CC-GUAUGGa---UGCGGUg -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 42275 | 0.66 | 0.752096 |
Target: 5'- cGCAuccGCcUGCGGgGaaGCCUgGCGCCACg -3' miRNA: 3'- aUGU---UGuACGCCgUa-UGGA-UGCGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 16850 | 0.66 | 0.74106 |
Target: 5'- gGCcGCAgaccGCGGCGUACaacuccgACGCCGa -3' miRNA: 3'- aUGuUGUa---CGCCGUAUGga-----UGCGGUg -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 3532 | 0.66 | 0.74106 |
Target: 5'- aGCGACGUGCcGUu--CCagGCGCCGCc -3' miRNA: 3'- aUGUUGUACGcCGuauGGa-UGCGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 11198 | 0.66 | 0.74106 |
Target: 5'- --gAugGUGCGGUcaAUGCCgcgcaugacCGCCACg -3' miRNA: 3'- augUugUACGCCG--UAUGGau-------GCGGUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 33732 | 0.66 | 0.74106 |
Target: 5'- gGCAGCAaucugcGCGGCGcacACCUGgGCgGCg -3' miRNA: 3'- aUGUUGUa-----CGCCGUa--UGGAUgCGgUG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 1747 | 0.66 | 0.74106 |
Target: 5'- aACAcCggGCGGuCAUggccgGCCUGCGCCugGCg -3' miRNA: 3'- aUGUuGuaCGCC-GUA-----UGGAUGCGG--UG- -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 21705 | 0.66 | 0.737724 |
Target: 5'- aGCAGCAUGuCGGCcugauggggugcccGUGCCU-CGUCGu -3' miRNA: 3'- aUGUUGUAC-GCCG--------------UAUGGAuGCGGUg -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 33960 | 0.66 | 0.729899 |
Target: 5'- -uCGGC-UGCGGCAUcgcaggccACCUcgACGCCGa -3' miRNA: 3'- auGUUGuACGCCGUA--------UGGA--UGCGGUg -5' |
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26623 | 3' | -52.4 | NC_005357.1 | + | 8778 | 0.66 | 0.727654 |
Target: 5'- gGCAGCAcGCGGCGcacgaugggucgGCC-GCGCCGg -3' miRNA: 3'- aUGUUGUaCGCCGUa-----------UGGaUGCGGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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