Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26636 | 3' | -57.7 | NC_005808.1 | + | 7157 | 0.66 | 0.530739 |
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Target: 5'- -gUGCGCGUGG-GC-GCgCCGugGguAGGc -3' miRNA: 3'- cgACGCGCACCaCGaUG-GGCugC--UCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 4124 | 0.66 | 0.530739 |
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Target: 5'- cGCUGCaGCGU--UGCUuCCCGGCGcauaguGGa -3' miRNA: 3'- -CGACG-CGCAccACGAuGGGCUGCu-----CC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 25811 | 0.66 | 0.541311 |
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Target: 5'- aGCaGCGCGcccaGcGUGCUGCCgcCGGUGAGGa -3' miRNA: 3'- -CGaCGCGCa---C-CACGAUGG--GCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 10650 | 0.66 | 0.551953 |
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Target: 5'- gGCUGCGCGccugGGUaCUACgCC--CGAGGc -3' miRNA: 3'- -CGACGCGCa---CCAcGAUG-GGcuGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 4811 | 0.66 | 0.551954 |
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Target: 5'- cGCUGCGgGau-UGCUGgCCGGCGAcaGGu -3' miRNA: 3'- -CGACGCgCaccACGAUgGGCUGCU--CC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 31306 | 0.66 | 0.562658 |
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Target: 5'- gGCgacCGCGUGGUG--GCCCGGCu-GGa -3' miRNA: 3'- -CGac-GCGCACCACgaUGGGCUGcuCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 1708 | 0.66 | 0.562658 |
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Target: 5'- --cGC-CGUGGgccgGCgugCCGGCGAGGg -3' miRNA: 3'- cgaCGcGCACCa---CGaugGGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 30576 | 0.66 | 0.562658 |
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Target: 5'- aGCUGCGCcaccucGUGCUcaACCuCGACGAc- -3' miRNA: 3'- -CGACGCGcac---CACGA--UGG-GCUGCUcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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