Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26636 | 3' | -57.7 | NC_005808.1 | + | 28411 | 0.7 | 0.3416 |
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Target: 5'- --cGCGC-----GCUGCCCGACGAGGc -3' miRNA: 3'- cgaCGCGcaccaCGAUGGGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 34616 | 0.71 | 0.287911 |
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Target: 5'- --cGC-CGUGGUGCUgcuGCCUGACGAa- -3' miRNA: 3'- cgaCGcGCACCACGA---UGGGCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 12523 | 0.71 | 0.273841 |
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Target: 5'- --aGCGCGUcgaGGUGCUGCUCGACcucGGc -3' miRNA: 3'- cgaCGCGCA---CCACGAUGGGCUGcu-CC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 23929 | 0.72 | 0.223041 |
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Target: 5'- gGCauagGCGUGaUGGUcagGCgccGCCCGGCGAGGu -3' miRNA: 3'- -CGa---CGCGC-ACCA---CGa--UGGGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 14801 | 0.78 | 0.090178 |
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Target: 5'- uGCUGCGCGUacugccuGGgGCUACCgGGCGcAGGg -3' miRNA: 3'- -CGACGCGCA-------CCaCGAUGGgCUGC-UCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 25138 | 0.79 | 0.080733 |
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Target: 5'- cGCUGCGCuggGUGGcgaUGCUGCCCGAUGGu- -3' miRNA: 3'- -CGACGCG---CACC---ACGAUGGGCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 39773 | 0.81 | 0.054047 |
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Target: 5'- cGCUGCGCGUGGccGCgagcaccugGCCCGACGAa- -3' miRNA: 3'- -CGACGCGCACCa-CGa--------UGGGCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 2283 | 1.12 | 0.000266 |
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Target: 5'- uGCUGCGCGUGGUGCUACCCGACGAGGg -3' miRNA: 3'- -CGACGCGCACCACGAUGGGCUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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