Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26636 | 3' | -57.7 | NC_005808.1 | + | 27852 | 0.68 | 0.43969 |
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Target: 5'- gGCUGCGCGgcgcgcgcuucgUGuacGUGUccgaGCCgGACGAGGg -3' miRNA: 3'- -CGACGCGC------------AC---CACGa---UGGgCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 28411 | 0.7 | 0.3416 |
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Target: 5'- --cGCGC-----GCUGCCCGACGAGGc -3' miRNA: 3'- cgaCGCGcaccaCGAUGGGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 30277 | 0.68 | 0.43969 |
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Target: 5'- --aGUGCGUGcgcGUGCUcaaGCCCGGCGGc- -3' miRNA: 3'- cgaCGCGCAC---CACGA---UGGGCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 30576 | 0.66 | 0.562658 |
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Target: 5'- aGCUGCGCcaccucGUGCUcaACCuCGACGAc- -3' miRNA: 3'- -CGACGCGcac---CACGA--UGG-GCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 31306 | 0.66 | 0.562658 |
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Target: 5'- gGCgacCGCGUGGUG--GCCCGGCu-GGa -3' miRNA: 3'- -CGac-GCGCACCACgaUGGGCUGcuCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 34616 | 0.71 | 0.287911 |
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Target: 5'- --cGC-CGUGGUGCUgcuGCCUGACGAa- -3' miRNA: 3'- cgaCGcGCACCACGA---UGGGCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 36042 | 0.67 | 0.449381 |
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Target: 5'- aGCggGCGCGUGGcGCUGCgCG-CGGa- -3' miRNA: 3'- -CGa-CGCGCACCaCGAUGgGCuGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 39773 | 0.81 | 0.054047 |
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Target: 5'- cGCUGCGCGUGGccGCgagcaccugGCCCGACGAa- -3' miRNA: 3'- -CGACGCGCACCa-CGa--------UGGGCUGCUcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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