Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26636 | 3' | -57.7 | NC_005808.1 | + | 20687 | 0.67 | 0.47813 |
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Target: 5'- -aUGCGCGcacucuaUGG-GCUGgaCGACGAGGc -3' miRNA: 3'- cgACGCGC-------ACCaCGAUggGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 5469 | 0.67 | 0.449382 |
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Target: 5'- cGCcGgGCGgcgaGGUGUUG-CCGAUGGGGg -3' miRNA: 3'- -CGaCgCGCa---CCACGAUgGGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 1670 | 0.68 | 0.439691 |
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Target: 5'- uUUGCGCGUGaUGCUGCC--GCGcAGGu -3' miRNA: 3'- cGACGCGCACcACGAUGGgcUGC-UCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 16644 | 0.68 | 0.411352 |
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Target: 5'- --gGUGCa-GGUGCcGgCCGACGAGGg -3' miRNA: 3'- cgaCGCGcaCCACGaUgGGCUGCUCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 16117 | 0.69 | 0.366729 |
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Target: 5'- gGCUGCaCGUcGGUGgc-CCCGACGuAGGa -3' miRNA: 3'- -CGACGcGCA-CCACgauGGGCUGC-UCC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 34616 | 0.71 | 0.287911 |
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Target: 5'- --cGC-CGUGGUGCUgcuGCCUGACGAa- -3' miRNA: 3'- cgaCGcGCACCACGA---UGGGCUGCUcc -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 12523 | 0.71 | 0.273841 |
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Target: 5'- --aGCGCGUcgaGGUGCUGCUCGACcucGGc -3' miRNA: 3'- cgaCGCGCA---CCACGAUGGGCUGcu-CC- -5' |
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| 26636 | 3' | -57.7 | NC_005808.1 | + | 23929 | 0.72 | 0.223041 |
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Target: 5'- gGCauagGCGUGaUGGUcagGCgccGCCCGGCGAGGu -3' miRNA: 3'- -CGa---CGCGC-ACCA---CGa--UGGGCUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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