miRNA: 3'- -CGUUGaCCacgaGCUGUUCUACGACg -5'
miRNA: 3'- -CGUUGac-CACGAGcu-GUUCUACGA--CG- -5'
miRNA: 3'- -CGUUGa-CCACGA--GCUGuuCU----------ACGACG- -5'
miRNA: 3'- -CGUuGaCCACG---AgCUGUUCuaCGACG- -5'
miRNA: 3'- -CGU-UGACCACga--GCU-GUUCUACGACG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26692 | 3' | -50.6 | NC_005808.1 | + | 21454 | 0.67 | 0.809169 |
|
Target: 5'- uGCGGCgGGgg--CGGCAGGAUGUUGg -3' miRNA: 3'- -CGUUGaCCacgaGCUGUUCUACGACg -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 3282 | 0.67 | 0.809169 |
|
Target: 5'- cGCAACgccGUGCUUccuCGGGAUGCUuuGCg -3' miRNA: 3'- -CGUUGac-CACGAGcu-GUUCUACGA--CG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 5474 | 0.66 | 0.847422 |
|
Target: 5'- gGCGGCgaGGUGUUgcCGAUggGGgguacggcgaUGCUGCc -3' miRNA: 3'- -CGUUGa-CCACGA--GCUGuuCU----------ACGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 19481 | 0.66 | 0.873495 |
|
Target: 5'- uGCGcCaGGUGCcggUgGGCAGGccGCUGCg -3' miRNA: 3'- -CGUuGaCCACG---AgCUGUUCuaCGACG- -5' |
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| 26692 | 3' | -50.6 | NC_005808.1 | + | 32527 | 0.78 | 0.24506 |
|
Target: 5'- uGCAgGCUGGUGaugcCGGcCAGGAUGCUGCc -3' miRNA: 3'- -CGU-UGACCACga--GCU-GUUCUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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