Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26694 | 5' | -62.4 | NC_005808.1 | + | 23934 | 0.71 | 0.110125 |
|
Target: 5'- aGGCgUGauggucaGGCGCCGCCcGGCGAGGu- -3' miRNA: 3'- -CUGgACg------CUGCGGCGGaCCGCUCCuu -5' |
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| 26694 | 5' | -62.4 | NC_005808.1 | + | 27779 | 0.66 | 0.286321 |
|
Target: 5'- cGACCUGgGcgaaggcaGCGCCGCCgaGGUGcAGGu- -3' miRNA: 3'- -CUGGACgC--------UGCGGCGGa-CCGC-UCCuu -5' |
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| 26694 | 5' | -62.4 | NC_005808.1 | + | 33598 | 0.7 | 0.149034 |
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Target: 5'- cGCCUGCuggaaGCGCCcCCgcgcGGCGAGGAAa -3' miRNA: 3'- cUGGACGc----UGCGGcGGa---CCGCUCCUU- -5' |
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| 26694 | 5' | -62.4 | NC_005808.1 | + | 37728 | 0.72 | 0.101291 |
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Target: 5'- --aUUGCGugGCuCGCC-GGCGAGGAu -3' miRNA: 3'- cugGACGCugCG-GCGGaCCGCUCCUu -5' |
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| 26694 | 5' | -62.4 | NC_005808.1 | + | 38099 | 0.68 | 0.184801 |
|
Target: 5'- cGCCUGUGGCGCCGCgCgcauccGGCGGuuucgucguucuGGAAg -3' miRNA: 3'- cUGGACGCUGCGGCG-Ga-----CCGCU------------CCUU- -5' |
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| 26694 | 5' | -62.4 | NC_005808.1 | + | 38734 | 0.69 | 0.157337 |
|
Target: 5'- cGGCCUacaGCGACGgCGCCUGGUacaacuucgccGAGGu- -3' miRNA: 3'- -CUGGA---CGCUGCgGCGGACCG-----------CUCCuu -5' |
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| 26694 | 5' | -62.4 | NC_005808.1 | + | 42451 | 0.7 | 0.147824 |
|
Target: 5'- cGCCUGCGGgGaaGCCUGGCGccacgccguagcuuGGGAc -3' miRNA: 3'- cUGGACGCUgCggCGGACCGC--------------UCCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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