Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26694 | 5' | -62.4 | NC_005808.1 | + | 11693 | 0.74 | 0.068233 |
Target: 5'- aGACUgcgGCGGacuuaGCCGCUUGGCGAGGu- -3' miRNA: 3'- -CUGGa--CGCUg----CGGCGGACCGCUCCuu -5' |
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26694 | 5' | -62.4 | NC_005808.1 | + | 9315 | 0.67 | 0.216412 |
Target: 5'- cGACCUgGCGGCGCaCGUCggcgcGGCGGGcGGc -3' miRNA: 3'- -CUGGA-CGCUGCG-GCGGa----CCGCUC-CUu -5' |
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26694 | 5' | -62.4 | NC_005808.1 | + | 8471 | 0.69 | 0.157337 |
Target: 5'- uGGCCUGCGAUGCCGCagccgacGCGAGu-- -3' miRNA: 3'- -CUGGACGCUGCGGCGgac----CGCUCcuu -5' |
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26694 | 5' | -62.4 | NC_005808.1 | + | 7556 | 0.68 | 0.184801 |
Target: 5'- cGAUCUGCGGCGCCaCC-GGCuuGGAc -3' miRNA: 3'- -CUGGACGCUGCGGcGGaCCGcuCCUu -5' |
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26694 | 5' | -62.4 | NC_005808.1 | + | 5353 | 0.7 | 0.141132 |
Target: 5'- cGCgCUGCGugGCCGCCUGcaccGCGcccAGGGu -3' miRNA: 3'- cUG-GACGCugCGGCGGAC----CGC---UCCUu -5' |
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26694 | 5' | -62.4 | NC_005808.1 | + | 5146 | 0.66 | 0.29349 |
Target: 5'- uGCCgcgcagGCGGCcGUCGCUgcucacgcccuUGGCGAGGGu -3' miRNA: 3'- cUGGa-----CGCUG-CGGCGG-----------ACCGCUCCUu -5' |
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26694 | 5' | -62.4 | NC_005808.1 | + | 4785 | 0.66 | 0.27929 |
Target: 5'- cACaCUGCGcccagcACGCCGgCUGGCGcugcGGGAu -3' miRNA: 3'- cUG-GACGC------UGCGGCgGACCGC----UCCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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