Results 1 - 20 of 94 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26699 | 5' | -59.5 | NC_005808.1 | + | 41647 | 0.66 | 0.427426 |
Target: 5'- aCGUGGCUCGAcacGCAgAGCGUGGagaauuCGACCa -3' miRNA: 3'- -GCGCUGGGCU---CGU-UCGCGCU------GCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41789 | 0.66 | 0.446353 |
Target: 5'- -uCGGCaaGGGCuGGGCGCGGCGcgucGCCGg -3' miRNA: 3'- gcGCUGggCUCG-UUCGCGCUGC----UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 16649 | 0.66 | 0.446353 |
Target: 5'- aCGaCGGCUacaaGGuCAAGCGCGGCGACa- -3' miRNA: 3'- -GC-GCUGGg---CUcGUUCGCGCUGCUGgc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 22389 | 0.66 | 0.436831 |
Target: 5'- aGcCGGCCgCGGGCGcugccaagaccgAGgGCGACGACa- -3' miRNA: 3'- gC-GCUGG-GCUCGU------------UCgCGCUGCUGgc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 31526 | 0.66 | 0.427426 |
Target: 5'- gCGCGACCCggacacguucGAGCAGuucGuCGCcaauGCGGCCGa -3' miRNA: 3'- -GCGCUGGG----------CUCGUU---C-GCGc---UGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 34909 | 0.66 | 0.475594 |
Target: 5'- aGcCGGCcgCCGAGguCAAGC-CGACGACCu -3' miRNA: 3'- gC-GCUG--GGCUC--GUUCGcGCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 37218 | 0.66 | 0.464759 |
Target: 5'- gCGCGcaccagugacauaACCCuGGGCGcGGUGCaGGCGGCCa -3' miRNA: 3'- -GCGC-------------UGGG-CUCGU-UCGCG-CUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 4875 | 0.66 | 0.465739 |
Target: 5'- uCGUuGCCC-AGCGcGGCGaCGGCGGCCu -3' miRNA: 3'- -GCGcUGGGcUCGU-UCGC-GCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 23569 | 0.66 | 0.465739 |
Target: 5'- uCGcCGGCaggugCGGGCGgggGGCGaCGAUGGCCGu -3' miRNA: 3'- -GC-GCUGg----GCUCGU---UCGC-GCUGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 19874 | 0.66 | 0.455991 |
Target: 5'- gGCGugCCGAagGCGucGCGCGccuucuUGGCCGu -3' miRNA: 3'- gCGCugGGCU--CGUu-CGCGCu-----GCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 2910 | 0.66 | 0.446353 |
Target: 5'- gCGCGcCCCGAuGUucGCGUucgaguucGACGGCCc -3' miRNA: 3'- -GCGCuGGGCU-CGuuCGCG--------CUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 32914 | 0.66 | 0.475594 |
Target: 5'- uGCGGCCaCG-GCcuGCaCGGCGGCUGu -3' miRNA: 3'- gCGCUGG-GCuCGuuCGcGCUGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 7352 | 0.66 | 0.474604 |
Target: 5'- gCGCGGCCucgaccucggcguCGuucguccagcGGCGGGCaccGCGGCGACCu -3' miRNA: 3'- -GCGCUGG-------------GC----------UCGUUCG---CGCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 18895 | 0.66 | 0.436831 |
Target: 5'- cCGCGACCUGGGCGcuaccGGCcuguacGUGuccggccCGACCGa -3' miRNA: 3'- -GCGCUGGGCUCGU-----UCG------CGCu------GCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 16332 | 0.66 | 0.455991 |
Target: 5'- gCGCGAgCCGAcCAGGC-CGGCGGgugcCCGu -3' miRNA: 3'- -GCGCUgGGCUcGUUCGcGCUGCU----GGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 1316 | 0.66 | 0.427426 |
Target: 5'- gGCGACggccaccaggCCGA-CAAGCGCGAgGaaGCCGu -3' miRNA: 3'- gCGCUG----------GGCUcGUUCGCGCUgC--UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 27827 | 0.66 | 0.427426 |
Target: 5'- gCGCGcuGCCCGcGguGGCGC--CGGCCGc -3' miRNA: 3'- -GCGC--UGGGCuCguUCGCGcuGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 29692 | 0.66 | 0.455991 |
Target: 5'- gCGCGG-CCGAGCGcaucgccaaAG-GCGACGACa- -3' miRNA: 3'- -GCGCUgGGCUCGU---------UCgCGCUGCUGgc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 41914 | 0.66 | 0.426492 |
Target: 5'- gGCG-CCCG-GCcGGCGCcgccguaGGCGGCCu -3' miRNA: 3'- gCGCuGGGCuCGuUCGCG-------CUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 33962 | 0.66 | 0.474604 |
Target: 5'- gCGCGGCCUgGGGCGuuuccgaAGgGCGuacCGGCCa -3' miRNA: 3'- -GCGCUGGG-CUCGU-------UCgCGCu--GCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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