Results 1 - 20 of 94 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26699 | 5' | -59.5 | NC_005808.1 | + | 21947 | 0.67 | 0.399948 |
Target: 5'- --aGGCCCaacgGGGCAcaguGCGCGgaACGACCGa -3' miRNA: 3'- gcgCUGGG----CUCGUu---CGCGC--UGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 32680 | 0.69 | 0.31692 |
Target: 5'- aCGUGaaGCCCGAGCAGuucGC-CGACGugCu -3' miRNA: 3'- -GCGC--UGGGCUCGUU---CGcGCUGCugGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 36198 | 0.68 | 0.356741 |
Target: 5'- uCGaCGACaucaCCGAGgGcGcCGCGGCGGCCGa -3' miRNA: 3'- -GC-GCUG----GGCUCgUuC-GCGCUGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 25597 | 0.68 | 0.362589 |
Target: 5'- uGCGGCCCaGGCAguggcaaccacggcGGCGCGGuCG-CCa -3' miRNA: 3'- gCGCUGGGcUCGU--------------UCGCGCU-GCuGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 27418 | 0.68 | 0.365116 |
Target: 5'- uCGCGcGCCUGGcGCAccGCGCccGGCGGCCGc -3' miRNA: 3'- -GCGC-UGGGCU-CGUu-CGCG--CUGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 39656 | 0.67 | 0.373625 |
Target: 5'- uCGaCGugCCGcGCGAcGCgGUGugGGCCGg -3' miRNA: 3'- -GC-GCugGGCuCGUU-CG-CGCugCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 8842 | 0.67 | 0.382268 |
Target: 5'- cCGCaACgCGGGCcAGCGCGuuGGCCu -3' miRNA: 3'- -GCGcUGgGCUCGuUCGCGCugCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 22233 | 0.67 | 0.391043 |
Target: 5'- aCGCGGCCCuGGUugucGGcCGCGAUGGCg- -3' miRNA: 3'- -GCGCUGGGcUCGu---UC-GCGCUGCUGgc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 28788 | 0.67 | 0.396371 |
Target: 5'- uCGCGcuuGCCCugguugccgaucuuGAGCAgccAGCGCGccgucgucggguuGCGGCCGg -3' miRNA: 3'- -GCGC---UGGG--------------CUCGU---UCGCGC-------------UGCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 36112 | 0.69 | 0.309369 |
Target: 5'- gCGCGugaAUgCGGGCcuGCGCGGCGugCa -3' miRNA: 3'- -GCGC---UGgGCUCGuuCGCGCUGCugGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 24447 | 0.69 | 0.29396 |
Target: 5'- gCGCGGCCUcGGCGAcauugacGC-CGACGACCu -3' miRNA: 3'- -GCGCUGGGcUCGUU-------CGcGCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 28673 | 0.7 | 0.266945 |
Target: 5'- gGCGGCCUc-GCcAGCGCGGCGcGCCa -3' miRNA: 3'- gCGCUGGGcuCGuUCGCGCUGC-UGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 3465 | 0.72 | 0.186278 |
Target: 5'- gCGCGcCCCGAuguucGCGGGCGaguuCGACGGCCc -3' miRNA: 3'- -GCGCuGGGCU-----CGUUCGC----GCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 10936 | 0.71 | 0.212287 |
Target: 5'- uGCagGGCCuCGGGCAGuuGCGCGGCGACa- -3' miRNA: 3'- gCG--CUGG-GCUCGUU--CGCGCUGCUGgc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 42063 | 0.71 | 0.216168 |
Target: 5'- aGCGGCCCGcGCAcguccgagguauucGGCcaGCGAUGGCCc -3' miRNA: 3'- gCGCUGGGCuCGU--------------UCG--CGCUGCUGGc -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 32518 | 0.71 | 0.23529 |
Target: 5'- cCGCG-CCCGuGCAGGCugGUGAUG-CCGg -3' miRNA: 3'- -GCGCuGGGCuCGUUCG--CGCUGCuGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 11393 | 0.7 | 0.247558 |
Target: 5'- gGCGACCUGAuggGCcagggucuuGAGCaGCGGCGAgCCGg -3' miRNA: 3'- gCGCUGGGCU---CG---------UUCG-CGCUGCU-GGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 39779 | 0.7 | 0.253887 |
Target: 5'- gCGUGGCCgCGAGCAccuGGCcCGACGaAUCGa -3' miRNA: 3'- -GCGCUGG-GCUCGU---UCGcGCUGC-UGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 22085 | 0.7 | 0.260349 |
Target: 5'- gCGCGGCUCGAu--GGCGUGAaUGACCGu -3' miRNA: 3'- -GCGCUGGGCUcguUCGCGCU-GCUGGC- -5' |
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26699 | 5' | -59.5 | NC_005808.1 | + | 15709 | 0.7 | 0.26628 |
Target: 5'- gGCGuccGCCUGGGCAucgaacuGGCGCGA-GGCCa -3' miRNA: 3'- gCGC---UGGGCUCGU-------UCGCGCUgCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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