Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26706 | 3' | -53.1 | NC_005808.1 | + | 22322 | 0.67 | 0.732433 |
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Target: 5'- -aCUGGUGCGGG-CAACGAcgGCCa-- -3' miRNA: 3'- cgGGCCACGUCUaGUUGCUa-UGGcuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 19037 | 0.67 | 0.736762 |
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Target: 5'- aGCCCuGGUaGCGgccgccggcggcgucGAUCAGCGuguccucGCCGAGg -3' miRNA: 3'- -CGGG-CCA-CGU---------------CUAGUUGCua-----UGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 632 | 0.67 | 0.743222 |
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Target: 5'- aGCCuCGGcGCGGGUgAACGGgucagGCgGGGg -3' miRNA: 3'- -CGG-GCCaCGUCUAgUUGCUa----UGgCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 23160 | 0.66 | 0.750704 |
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Target: 5'- aGCCCGagaagucccgcaccGUGCAGGUCAaggacagcaugGgGAUGCUGGa -3' miRNA: 3'- -CGGGC--------------CACGUCUAGU-----------UgCUAUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 3741 | 0.66 | 0.774825 |
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Target: 5'- -gCCGGUGguGggCAcguCGGUGCCGc- -3' miRNA: 3'- cgGGCCACguCuaGUu--GCUAUGGCuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 38514 | 0.66 | 0.785065 |
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Target: 5'- aCCaCGGcGCAGAUCAACGAauacaGCCu-- -3' miRNA: 3'- cGG-GCCaCGUCUAGUUGCUa----UGGcuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 18944 | 0.66 | 0.795137 |
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Target: 5'- cGCCUGGUGCAGuugCugcCGccGCCGGc -3' miRNA: 3'- -CGGGCCACGUCua-Guu-GCuaUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 38105 | 0.66 | 0.795137 |
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Target: 5'- cGCCC-GUG-AGAUUAccguCGAUACCGAc -3' miRNA: 3'- -CGGGcCACgUCUAGUu---GCUAUGGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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