Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26706 | 3' | -53.1 | NC_005808.1 | + | 31575 | 0.71 | 0.499097 |
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Target: 5'- gGCCCGGUGCAGcagguguUCAuCGAcGCCa-- -3' miRNA: 3'- -CGGGCCACGUCu------AGUuGCUaUGGcuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 35222 | 0.71 | 0.488504 |
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Target: 5'- cGCCgCGGUGCccgccgcuGGAcgAACGAcGCCGAGg -3' miRNA: 3'- -CGG-GCCACG--------UCUagUUGCUaUGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 11702 | 0.71 | 0.478017 |
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Target: 5'- aUCCGGUGCAGuGUCGGCugggauAUGCCGAc -3' miRNA: 3'- cGGGCCACGUC-UAGUUGc-----UAUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 24547 | 0.71 | 0.467643 |
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Target: 5'- cGCgUGGUcgcGCAGcgCGGCGAUGgCGAGg -3' miRNA: 3'- -CGgGCCA---CGUCuaGUUGCUAUgGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 32521 | 0.72 | 0.437241 |
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Target: 5'- cGCCC-GUGCAGGcUggUGAUGCCGGc -3' miRNA: 3'- -CGGGcCACGUCUaGuuGCUAUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 15387 | 0.72 | 0.408005 |
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Target: 5'- cGUCCGcGUGCGGGcCGGCGAagaUGCCGGa -3' miRNA: 3'- -CGGGC-CACGUCUaGUUGCU---AUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 29964 | 0.73 | 0.380024 |
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Target: 5'- cGCCaaaGGacgGCAG--CAGCGAUGCCGAGc -3' miRNA: 3'- -CGGg--CCa--CGUCuaGUUGCUAUGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 16789 | 1.1 | 0.00112 |
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Target: 5'- gGCCCGGUGCAGAUCAACGAUACCGAGc -3' miRNA: 3'- -CGGGCCACGUCUAGUUGCUAUGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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