Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26706 | 3' | -53.1 | NC_005808.1 | + | 32853 | 0.69 | 0.575703 |
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Target: 5'- cGCCUGGccGCAGA-CGGuCGGcGCCGAGa -3' miRNA: 3'- -CGGGCCa-CGUCUaGUU-GCUaUGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 33784 | 0.67 | 0.710551 |
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Target: 5'- gGCCCGacccaGCAGAUCGguacugaccgGCGcgGCCGAc -3' miRNA: 3'- -CGGGCca---CGUCUAGU----------UGCuaUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 35047 | 0.67 | 0.688343 |
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Target: 5'- -gCCGGUggcgccGCAGAUCGagcACGccGCCGAGc -3' miRNA: 3'- cgGGCCA------CGUCUAGU---UGCuaUGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 35222 | 0.71 | 0.488504 |
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Target: 5'- cGCCgCGGUGCccgccgcuGGAcgAACGAcGCCGAGg -3' miRNA: 3'- -CGG-GCCACG--------UCUagUUGCUaUGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 37242 | 0.68 | 0.665906 |
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Target: 5'- gGCgCGGUGCAGGcggccacgCAgcGCGAUGCCa-- -3' miRNA: 3'- -CGgGCCACGUCUa-------GU--UGCUAUGGcuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 38105 | 0.66 | 0.795137 |
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Target: 5'- cGCCC-GUG-AGAUUAccguCGAUACCGAc -3' miRNA: 3'- -CGGGcCACgUCUAGUu---GCUAUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 38514 | 0.66 | 0.785065 |
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Target: 5'- aCCaCGGcGCAGAUCAACGAauacaGCCu-- -3' miRNA: 3'- cGG-GCCaCGUCUAGUUGCUa----UGGcuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 42409 | 0.68 | 0.654633 |
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Target: 5'- cGCCCGGcagcgUGaCGGugccCAGCGAcACCGGGg -3' miRNA: 3'- -CGGGCC-----AC-GUCua--GUUGCUaUGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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