Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26706 | 3' | -53.1 | NC_005808.1 | + | 31100 | 0.68 | 0.63203 |
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Target: 5'- cCUCGGUGCAGGcCGGCGAguggugCGAGa -3' miRNA: 3'- cGGGCCACGUCUaGUUGCUaug---GCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 13467 | 0.68 | 0.664781 |
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Target: 5'- gGCgCGGUGuCGGGcaugaccUgGGCGAUGCCGAc -3' miRNA: 3'- -CGgGCCAC-GUCU-------AgUUGCUAUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 37242 | 0.68 | 0.665906 |
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Target: 5'- gGCgCGGUGCAGGcggccacgCAgcGCGAUGCCa-- -3' miRNA: 3'- -CGgGCCACGUCUa-------GU--UGCUAUGGcuc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 10941 | 0.68 | 0.665906 |
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Target: 5'- gGCCuCGG-GCAGuugcgCGGCGAcaGCCGGGg -3' miRNA: 3'- -CGG-GCCaCGUCua---GUUGCUa-UGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 23953 | 0.68 | 0.677147 |
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Target: 5'- cGCCCGGcG-AGGUCGGCauuUGCCGAu -3' miRNA: 3'- -CGGGCCaCgUCUAGUUGcu-AUGGCUc -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 35047 | 0.67 | 0.688343 |
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Target: 5'- -gCCGGUggcgccGCAGAUCGagcACGccGCCGAGc -3' miRNA: 3'- cgGGCCA------CGUCUAGU---UGCuaUGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 19037 | 0.67 | 0.736762 |
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Target: 5'- aGCCCuGGUaGCGgccgccggcggcgucGAUCAGCGuguccucGCCGAGg -3' miRNA: 3'- -CGGG-CCA-CGU---------------CUAGUUGCua-----UGGCUC- -5' |
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| 26706 | 3' | -53.1 | NC_005808.1 | + | 15387 | 0.72 | 0.408005 |
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Target: 5'- cGUCCGcGUGCGGGcCGGCGAagaUGCCGGa -3' miRNA: 3'- -CGGGC-CACGUCUaGUUGCU---AUGGCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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