miRNA: 3'- uaGC-GCCGGUAGCaaCGG--CGGCGGGU- -5'
miRNA: 3'- -UAGCGCCGG---UAGCAACgGCGG-CGG-GU- -5'
miRNA: 3'- -UAgCGCCGGUaGCAACgGCGGCGGgu -5'
miRNA: 3'- uagCGcCGGUAGcAACGGCGGCGGGU- -5'
miRNA: 3'- -UAGCGCCGGUAGCAACGGCGGCGGGU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26706 | 5' | -62.3 | NC_005808.1 | + | 11565 | 0.73 | 0.095518 |
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Target: 5'- -cCGcCGGUCAUCGaaGCCauGCCGCCCAg -3' miRNA: 3'- uaGC-GCCGGUAGCaaCGG--CGGCGGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 6414 | 0.73 | 0.092882 |
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Target: 5'- aGUCGCGGCCcauGUCGUUGgCGCCuGUCaCGg -3' miRNA: 3'- -UAGCGCCGG---UAGCAACgGCGG-CGG-GU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 37026 | 0.73 | 0.080709 |
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Target: 5'- gGUgGCGGCCAgCGUUGgCGCCGUCg- -3' miRNA: 3'- -UAgCGCCGGUaGCAACgGCGGCGGgu -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 8038 | 0.74 | 0.070069 |
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Target: 5'- --gGCcGCCGUCcUUGCCGCCGUCCAu -3' miRNA: 3'- uagCGcCGGUAGcAACGGCGGCGGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 16827 | 1.06 | 0.000221 |
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Target: 5'- cAUCGCGGCCAUCGUUGCCGCCGCCCAg -3' miRNA: 3'- -UAGCGCCGGUAGCAACGGCGGCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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