miRNA: 3'- -UAGCGCCGGUagCA------------ACGGCG-GCGGGU- -5'
miRNA: 3'- uAGCGCCGGU------------AGCaaCGgCGGCgGGU- -5'
miRNA: 3'- uaGCGC-CGGua----GCAACGGC-GGCGGGU- -5'
miRNA: 3'- uAGC-GCCGGuAgCAaCGGCGGCGGGu -5'
miRNA: 3'- uAGCGCCG--GUAgCaACGGCGGCgGGU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26706 | 5' | -62.3 | NC_005808.1 | + | 38172 | 0.69 | 0.169871 |
|
Target: 5'- uGUUGCGGCCGg-GUacgaccuacgacUGCCGCaCGCUCAa -3' miRNA: 3'- -UAGCGCCGGUagCA------------ACGGCG-GCGGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 38408 | 0.68 | 0.215406 |
|
Target: 5'- cUCGCGGCCAacaugcccgccaUCGagGCgGCCGgCUAc -3' miRNA: 3'- uAGCGCCGGU------------AGCaaCGgCGGCgGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 40228 | 0.66 | 0.291959 |
|
Target: 5'- -cCGUGcGCCucgaacCGUUGCCGuUUGCCCAg -3' miRNA: 3'- uaGCGC-CGGua----GCAACGGC-GGCGGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 41283 | 0.68 | 0.215406 |
|
Target: 5'- cUUGuCGGCC-UgGUgGCCGUCGCCCu -3' miRNA: 3'- uAGC-GCCGGuAgCAaCGGCGGCGGGu -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 41555 | 0.69 | 0.188942 |
|
Target: 5'- uUCGCGGCgaCAU-GcUGCCGCCGgCCGu -3' miRNA: 3'- uAGCGCCG--GUAgCaACGGCGGCgGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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