Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26706 | 5' | -62.3 | NC_005808.1 | + | 4651 | 0.66 | 0.289808 |
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Target: 5'- -gCGuCGGCCAUCGcgucaaggucgaUGCCguagguaGCCGCCCc -3' miRNA: 3'- uaGC-GCCGGUAGCa-----------ACGG-------CGGCGGGu -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 20266 | 0.66 | 0.29124 |
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Target: 5'- uGUUGCugcuguuGGCCGUCGUUGC--CCGCaCCAg -3' miRNA: 3'- -UAGCG-------CCGGUAGCAACGgcGGCG-GGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 40228 | 0.66 | 0.291959 |
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Target: 5'- -cCGUGcGCCucgaacCGUUGCCGuUUGCCCAg -3' miRNA: 3'- uaGCGC-CGGua----GCAACGGC-GGCGGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 12490 | 0.66 | 0.296297 |
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Target: 5'- -gCGCGGCCucAUCGccugggacaaggGCCGUggCGCCCGg -3' miRNA: 3'- uaGCGCCGG--UAGCaa----------CGGCG--GCGGGU- -5' |
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| 26706 | 5' | -62.3 | NC_005808.1 | + | 1832 | 0.65 | 0.303639 |
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Target: 5'- --aGUGGCCGaagaaagccaggaUGUUGCCGCCGUCUu -3' miRNA: 3'- uagCGCCGGUa------------GCAACGGCGGCGGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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