Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26740 | 5' | -64.2 | NC_005808.1 | + | 1503 | 0.72 | 0.097642 |
Target: 5'- uGCUGGuCGgacauggcgCC-CCCUuauUCGCCGCCCAg -3' miRNA: 3'- gCGGCC-GCa--------GGuGGGA---AGCGGCGGGU- -5' |
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26740 | 5' | -64.2 | NC_005808.1 | + | 1157 | 0.68 | 0.194552 |
Target: 5'- aCGgCGGCgGUCaCGCCCcacauagucUCGCCGCCgGg -3' miRNA: 3'- -GCgGCCG-CAG-GUGGGa--------AGCGGCGGgU- -5' |
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26740 | 5' | -64.2 | NC_005808.1 | + | 693 | 0.71 | 0.100332 |
Target: 5'- gCGCCGGCcgggCCGCCCaagauaCCGCCCAg -3' miRNA: 3'- -GCGGCCGca--GGUGGGaagc--GGCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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