miRNA: 3'- -GUGU----UUGUCaa-CGGG-CGaCAGCGGGG- -5'
miRNA: 3'- gUGUUUGUCa--AC-GGGCGaC-----AGCGGGG- -5'
miRNA: 3'- -GUGUU---UGUCAa-CGGGCGACAG---------------CGGGg -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26744 | 5' | -55 | NC_005808.1 | + | 35399 | 0.67 | 0.598668 |
|
Target: 5'- aCGCAguccGAUGGcaaGCCCaGC-GUCGCCCCc -3' miRNA: 3'- -GUGU----UUGUCaa-CGGG-CGaCAGCGGGG- -5' |
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| 26744 | 5' | -55 | NC_005808.1 | + | 39587 | 0.65 | 0.677324 |
|
Target: 5'- gACGAGCAGcuaUGaCCCGCcGcgccuUCGUCCCc -3' miRNA: 3'- gUGUUUGUCa--AC-GGGCGaC-----AGCGGGG- -5' |
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| 26744 | 5' | -55 | NC_005808.1 | + | 41755 | 0.68 | 0.547544 |
|
Target: 5'- aUACAGgccACGGUcgGCUCGCUGUCucugcgcgcgcucaaGCCCa -3' miRNA: 3'- -GUGUU---UGUCAa-CGGGCGACAG---------------CGGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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