Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26785 | 3' | -63.1 | NC_005808.1 | + | 34317 | 1.12 | 0.000066 |
Target: 5'- uGCCCCGGCAGCGUGGCCCUGGAAGCCg -3' miRNA: 3'- -CGGGGCCGUCGCACCGGGACCUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 27435 | 0.76 | 0.048811 |
Target: 5'- cGCgCCCGGCGGCcgcaGGCCCUGGcgcugguauGCCa -3' miRNA: 3'- -CG-GGGCCGUCGca--CCGGGACCuu-------CGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 20208 | 0.76 | 0.053726 |
Target: 5'- gGUCuuGGCAGCGcccgcggccggcuguUGGCCUUGGguGCCu -3' miRNA: 3'- -CGGggCCGUCGC---------------ACCGGGACCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 28586 | 0.75 | 0.064131 |
Target: 5'- gGCCaCCGGCAGCGUGGugucggucaguuCCUUGaaccgggcgcggauGAGGCCg -3' miRNA: 3'- -CGG-GGCCGUCGCACC------------GGGAC--------------CUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 13248 | 0.74 | 0.072325 |
Target: 5'- gGCCaCCGGC-GCGUGGUUCgaGGAcuuGGCCg -3' miRNA: 3'- -CGG-GGCCGuCGCACCGGGa-CCU---UCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 10457 | 0.74 | 0.07437 |
Target: 5'- cGCCCU--UGGCGUcGCCCUGGAAGUCg -3' miRNA: 3'- -CGGGGccGUCGCAcCGGGACCUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 31007 | 0.72 | 0.095436 |
Target: 5'- -gCCCGGCGGCcagccggcGGCCCUGGGcGGCa -3' miRNA: 3'- cgGGGCCGUCGca------CCGGGACCU-UCGg -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 13577 | 0.71 | 0.118794 |
Target: 5'- -aCCUGGCcgaAGCGcGGCgCCUGGucGCCu -3' miRNA: 3'- cgGGGCCG---UCGCaCCG-GGACCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 31235 | 0.71 | 0.118794 |
Target: 5'- aCCCCGGC-GCGcGGCCCaGcuacGAGCCg -3' miRNA: 3'- cGGGGCCGuCGCaCCGGGaCc---UUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 1534 | 0.7 | 0.143496 |
Target: 5'- cGCCCa-GCAGUucGGCCUUGGuaAAGCCg -3' miRNA: 3'- -CGGGgcCGUCGcaCCGGGACC--UUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 41303 | 0.7 | 0.155045 |
Target: 5'- cGCCCugggcgCGGCGGUGUGGgcgggcaucaCCUcgaaacuUGGggGCCa -3' miRNA: 3'- -CGGG------GCCGUCGCACC----------GGG-------ACCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 1952 | 0.69 | 0.161344 |
Target: 5'- gGUUCUGGUcgAGCGcgcGGCCCUGGGggaaggcacgcuguaGGCCg -3' miRNA: 3'- -CGGGGCCG--UCGCa--CCGGGACCU---------------UCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 25140 | 0.69 | 0.162632 |
Target: 5'- gGCCCUGGC---GUGGCaCCUGGccucgaugcuggcgGGGCCa -3' miRNA: 3'- -CGGGGCCGucgCACCG-GGACC--------------UUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 682 | 0.68 | 0.186939 |
Target: 5'- cGCCUaCGGCGGCGccGGCCg-GGccGCCc -3' miRNA: 3'- -CGGG-GCCGUCGCa-CCGGgaCCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 18367 | 0.68 | 0.188897 |
Target: 5'- uUgCCGGCGGCGagguUGGCCUugccgauggcgccggUGGcGGCCa -3' miRNA: 3'- cGgGGCCGUCGC----ACCGGG---------------ACCuUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 17918 | 0.68 | 0.191869 |
Target: 5'- aGCCUgGGCguGGaCGUGGCCCgcggcGGgcGCg -3' miRNA: 3'- -CGGGgCCG--UC-GCACCGGGa----CCuuCGg -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 12468 | 0.68 | 0.191869 |
Target: 5'- uGCaggCCGGCGGCGU-GUUCUGGcgcGGCCu -3' miRNA: 3'- -CGg--GGCCGUCGCAcCGGGACCu--UCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 19013 | 0.68 | 0.196914 |
Target: 5'- uGUCCCacuuguaguGGguGCGguaGCCCUGGuagcGGCCg -3' miRNA: 3'- -CGGGG---------CCguCGCac-CGGGACCu---UCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 42410 | 0.68 | 0.202074 |
Target: 5'- -gCCCGGCAGCGUGacgguGCCCagcgacaccgGGgcGCa -3' miRNA: 3'- cgGGGCCGUCGCAC-----CGGGa---------CCuuCGg -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 8166 | 0.68 | 0.202075 |
Target: 5'- cGUCCCuGC-GCGUGcGCCC-GGAugcuGCCg -3' miRNA: 3'- -CGGGGcCGuCGCAC-CGGGaCCUu---CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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