Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26785 | 3' | -63.1 | NC_005808.1 | + | 27706 | 0.67 | 0.256511 |
Target: 5'- cGCgCCGGCAGCGgcucgccgccggucaUGGaCUUGauGAGGCCc -3' miRNA: 3'- -CGgGGCCGUCGC---------------ACCgGGAC--CUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 32378 | 0.68 | 0.207352 |
Target: 5'- gGCCCCGGCc-----GCCCagcaGGAAGCCa -3' miRNA: 3'- -CGGGGCCGucgcacCGGGa---CCUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 21161 | 0.67 | 0.218264 |
Target: 5'- cGCCCUGGUucaacAGCGcGGCCUgcUGcGcGGCCu -3' miRNA: 3'- -CGGGGCCG-----UCGCaCCGGG--AC-CuUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 35425 | 0.67 | 0.223902 |
Target: 5'- cGCCCCcGaauccgacaAGCGcccGGCCCUGGucauccAGGCCg -3' miRNA: 3'- -CGGGGcCg--------UCGCa--CCGGGACC------UUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 11514 | 0.67 | 0.235549 |
Target: 5'- aGCgCCUGcGCuGGCGUgaGGCCCUGcGAGCg -3' miRNA: 3'- -CG-GGGC-CG-UCGCA--CCGGGACcUUCGg -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 22744 | 0.67 | 0.241561 |
Target: 5'- uGCCCCaGCAGaGUGgacuuGCCCaGGGugagGGCCg -3' miRNA: 3'- -CGGGGcCGUCgCAC-----CGGGaCCU----UCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 25538 | 0.67 | 0.2477 |
Target: 5'- cGCCgCCGGCAGCGcc-CCCgu--AGCCg -3' miRNA: 3'- -CGG-GGCCGUCGCaccGGGaccuUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 28777 | 0.67 | 0.2477 |
Target: 5'- cGCgCaCGGCGucGCGcuuGCCCUGGuuGCCg -3' miRNA: 3'- -CGgG-GCCGU--CGCac-CGGGACCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 14815 | 0.67 | 0.253335 |
Target: 5'- aCCUCGGCGGCGcUGccuucGCCCaggucgaugccguUGGcGGCCa -3' miRNA: 3'- cGGGGCCGUCGC-AC-----CGGG-------------ACCuUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 964 | 0.68 | 0.207352 |
Target: 5'- uGCCCaCGGCAGCuugcagccacuUGGCcgCCUGGcgguGCCg -3' miRNA: 3'- -CGGG-GCCGUCGc----------ACCG--GGACCuu--CGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 8166 | 0.68 | 0.202075 |
Target: 5'- cGUCCCuGC-GCGUGcGCCC-GGAugcuGCCg -3' miRNA: 3'- -CGGGGcCGuCGCAC-CGGGaCCUu---CGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 19013 | 0.68 | 0.196914 |
Target: 5'- uGUCCCacuuguaguGGguGCGguaGCCCUGGuagcGGCCg -3' miRNA: 3'- -CGGGG---------CCguCGCac-CGGGACCu---UCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 27435 | 0.76 | 0.048811 |
Target: 5'- cGCgCCCGGCGGCcgcaGGCCCUGGcgcugguauGCCa -3' miRNA: 3'- -CG-GGGCCGUCGca--CCGGGACCuu-------CGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 20208 | 0.76 | 0.053726 |
Target: 5'- gGUCuuGGCAGCGcccgcggccggcuguUGGCCUUGGguGCCu -3' miRNA: 3'- -CGGggCCGUCGC---------------ACCGGGACCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 28586 | 0.75 | 0.064131 |
Target: 5'- gGCCaCCGGCAGCGUGGugucggucaguuCCUUGaaccgggcgcggauGAGGCCg -3' miRNA: 3'- -CGG-GGCCGUCGCACC------------GGGAC--------------CUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 10457 | 0.74 | 0.07437 |
Target: 5'- cGCCCU--UGGCGUcGCCCUGGAAGUCg -3' miRNA: 3'- -CGGGGccGUCGCAcCGGGACCUUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 31235 | 0.71 | 0.118794 |
Target: 5'- aCCCCGGC-GCGcGGCCCaGcuacGAGCCg -3' miRNA: 3'- cGGGGCCGuCGCaCCGGGaCc---UUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 1534 | 0.7 | 0.143496 |
Target: 5'- cGCCCa-GCAGUucGGCCUUGGuaAAGCCg -3' miRNA: 3'- -CGGGgcCGUCGcaCCGGGACC--UUCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 682 | 0.68 | 0.186939 |
Target: 5'- cGCCUaCGGCGGCGccGGCCg-GGccGCCc -3' miRNA: 3'- -CGGG-GCCGUCGCa-CCGGgaCCuuCGG- -5' |
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26785 | 3' | -63.1 | NC_005808.1 | + | 18367 | 0.68 | 0.188897 |
Target: 5'- uUgCCGGCGGCGagguUGGCCUugccgauggcgccggUGGcGGCCa -3' miRNA: 3'- cGgGGCCGUCGC----ACCGGG---------------ACCuUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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