Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26797 | 5' | -58.3 | NC_005808.1 | + | 17682 | 0.66 | 0.400922 |
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Target: 5'- --gCUGGgcGUAGGCgaAGCGCCACUc -3' miRNA: 3'- auaGGCCaaCGUCCGg-UCGUGGUGGu -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 41316 | 0.66 | 0.400922 |
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Target: 5'- ---gCGG-UGUGGGCgGGCAUCACCu -3' miRNA: 3'- auagGCCaACGUCCGgUCGUGGUGGu -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 10472 | 0.66 | 0.410346 |
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Target: 5'- gAUUCGGggGCgucgAGGUCGGCAgCgGCCAg -3' miRNA: 3'- aUAGGCCaaCG----UCCGGUCGU-GgUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 4041 | 0.66 | 0.416066 |
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Target: 5'- --gCCGGccgcggcgagcgGCAGGCCAGCGgC-CCAg -3' miRNA: 3'- auaGGCCaa----------CGUCCGGUCGUgGuGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 17295 | 0.66 | 0.419906 |
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Target: 5'- --gCCGGUgGCAgGGCCGgccGCACgACCu -3' miRNA: 3'- auaGGCCAaCGU-CCGGU---CGUGgUGGu -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 14847 | 0.66 | 0.449376 |
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Target: 5'- --gCCGuugGC-GGCCAGCGCCGCa- -3' miRNA: 3'- auaGGCcaaCGuCCGGUCGUGGUGgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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