Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26797 | 5' | -58.3 | NC_005808.1 | + | 365 | 0.7 | 0.224453 |
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Target: 5'- cAUCCGGgugUGCuuGGCCugccgauugAGCGCgGCCAg -3' miRNA: 3'- aUAGGCCa--ACGu-CCGG---------UCGUGgUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 15387 | 0.71 | 0.207035 |
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Target: 5'- cGUCCGcgUGCGGGCCGGCGaagaUGCCGg -3' miRNA: 3'- aUAGGCcaACGUCCGGUCGUg---GUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 16043 | 0.72 | 0.166219 |
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Target: 5'- --gCCaGUUGUAGGCCAGUucgcgcacgugGCCGCCGc -3' miRNA: 3'- auaGGcCAACGUCCGGUCG-----------UGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 41906 | 0.74 | 0.129055 |
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Target: 5'- gUAUCuUGGgcGCccGGCCGGCGCCGCCGu -3' miRNA: 3'- -AUAG-GCCaaCGu-CCGGUCGUGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 10055 | 0.75 | 0.111557 |
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Target: 5'- aUcgCCGGcgGCAgcauccuGGCCGGCAUCACCAg -3' miRNA: 3'- -AuaGGCCaaCGU-------CCGGUCGUGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 37390 | 1.07 | 0.000397 |
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Target: 5'- gUAUCCGGUUGCAGGCCAGCACCACCAg -3' miRNA: 3'- -AUAGGCCAACGUCCGGUCGUGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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