Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26797 | 5' | -58.3 | NC_005808.1 | + | 5112 | 0.69 | 0.256233 |
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Target: 5'- cGUCCGG-UGC-GGCUGGCGCCGuuGa -3' miRNA: 3'- aUAGGCCaACGuCCGGUCGUGGUggU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 4041 | 0.66 | 0.416066 |
|
Target: 5'- --gCCGGccgcggcgagcgGCAGGCCAGCGgC-CCAg -3' miRNA: 3'- auaGGCCaa----------CGUCCGGUCGUgGuGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 3741 | 0.68 | 0.330688 |
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Target: 5'- --gCCGGUgguggGCAcGUCGGUGCCGCCGc -3' miRNA: 3'- auaGGCCAa----CGUcCGGUCGUGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 3530 | 0.68 | 0.299124 |
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Target: 5'- aGUUCGacgUGUAGuGCCAGCuGCCGCCGa -3' miRNA: 3'- aUAGGCca-ACGUC-CGGUCG-UGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 2823 | 0.67 | 0.355928 |
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Target: 5'- --aCCGGcugaccaccuaUUGaggaaAGGCCAGC-CCGCCAa -3' miRNA: 3'- auaGGCC-----------AACg----UCCGGUCGuGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 365 | 0.7 | 0.224453 |
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Target: 5'- cAUCCGGgugUGCuuGGCCugccgauugAGCGCgGCCAg -3' miRNA: 3'- aUAGGCCa--ACGu-CCGG---------UCGUGgUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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