Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26797 | 5' | -58.3 | NC_005808.1 | + | 3530 | 0.68 | 0.299124 |
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Target: 5'- aGUUCGacgUGUAGuGCCAGCuGCCGCCGa -3' miRNA: 3'- aUAGGCca-ACGUC-CGGUCG-UGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 40843 | 0.68 | 0.299124 |
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Target: 5'- --gCCaGgcGCAGGCCGGCcaugACCGCCc -3' miRNA: 3'- auaGGcCaaCGUCCGGUCG----UGGUGGu -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 19619 | 0.69 | 0.269943 |
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Target: 5'- cGUCCGGggucaUGCGGGCCGcGaCAUCgGCCAg -3' miRNA: 3'- aUAGGCCa----ACGUCCGGU-C-GUGG-UGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 23737 | 0.7 | 0.249594 |
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Target: 5'- --cUCGGUggcgcGCAGcagcgccauGCCGGCGCCACCGg -3' miRNA: 3'- auaGGCCAa----CGUC---------CGGUCGUGGUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 15387 | 0.71 | 0.207035 |
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Target: 5'- cGUCCGcgUGCGGGCCGGCGaagaUGCCGg -3' miRNA: 3'- aUAGGCcaACGUCCGGUCGUg---GUGGU- -5' |
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| 26797 | 5' | -58.3 | NC_005808.1 | + | 16043 | 0.72 | 0.166219 |
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Target: 5'- --gCCaGUUGUAGGCCAGUucgcgcacgugGCCGCCGc -3' miRNA: 3'- auaGGcCAACGUCCGGUCG-----------UGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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