Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26811 | 3' | -53.6 | NC_005808.1 | + | 24551 | 0.66 | 0.693549 |
Target: 5'- ---cGCGCCGguAAGGCCCCCaagcagcccGCC-GACa -3' miRNA: 3'- ucuuUGUGGU--UUUCGGGGG---------CGGaCUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 1741 | 0.76 | 0.208142 |
Target: 5'- uAGAugAACACCGGGcggucauggccGGCCUgCGCCUGGCg -3' miRNA: 3'- -UCU--UUGUGGUUU-----------UCGGGgGCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 39185 | 0.72 | 0.371054 |
Target: 5'- uAGcgGgGCCGAAAGgCCCCGCCaaGGCa -3' miRNA: 3'- -UCuuUgUGGUUUUCgGGGGCGGa-CUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 16682 | 0.67 | 0.636443 |
Target: 5'- uGGGcCACCGcAAGCUgcaagucgaaaCCCGCCUGAa -3' miRNA: 3'- uCUUuGUGGUuUUCGG-----------GGGCGGACUg -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 18883 | 0.67 | 0.636443 |
Target: 5'- cGAGGCACU---GGCCCgCGaCCUGGg -3' miRNA: 3'- uCUUUGUGGuuuUCGGGgGC-GGACUg -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 20107 | 0.71 | 0.428337 |
Target: 5'- cGGGcAugACCAGcccAGCCCgccccugguuCCGCCUGACa -3' miRNA: 3'- -UCU-UugUGGUUu--UCGGG----------GGCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 21979 | 0.69 | 0.523153 |
Target: 5'- cAGGccGACACCGAccuGCgaCgCCGCCUGGCg -3' miRNA: 3'- -UCU--UUGUGGUUuu-CGg-G-GGCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 10123 | 0.68 | 0.579213 |
Target: 5'- cGGgcGCAUCGGccGCCCCCaCCUcGGCa -3' miRNA: 3'- -UCuuUGUGGUUuuCGGGGGcGGA-CUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 29270 | 0.66 | 0.670814 |
Target: 5'- cGGcGACcuGCCGAAAGCCUacgCCGCCU-ACa -3' miRNA: 3'- -UCuUUG--UGGUUUUCGGG---GGCGGAcUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 36204 | 0.7 | 0.479854 |
Target: 5'- cGAcuACACCGGGccggccGGCCUgCGCCUGAUu -3' miRNA: 3'- uCUu-UGUGGUUU------UCGGGgGCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 28228 | 0.7 | 0.458868 |
Target: 5'- cGcgGCGCgGAu-GCCCCCGCCUuccagGACa -3' miRNA: 3'- uCuuUGUGgUUuuCGGGGGCGGA-----CUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 23208 | 0.67 | 0.636443 |
Target: 5'- uGGAAaccuACGCCGAAguggacaaGGCCCUgGCC-GACc -3' miRNA: 3'- -UCUU----UGUGGUUU--------UCGGGGgCGGaCUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 35788 | 0.67 | 0.647919 |
Target: 5'- cGAAacccGCGCCGu--GUCCCUGgCUGACc -3' miRNA: 3'- uCUU----UGUGGUuuuCGGGGGCgGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 10689 | 0.69 | 0.512177 |
Target: 5'- cGAAACGCCGcAGGCaCUCgGCCUcGAUg -3' miRNA: 3'- uCUUUGUGGUuUUCG-GGGgCGGA-CUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 35088 | 0.66 | 0.727137 |
Target: 5'- -cGGACGCCGAAAGCgCgCGCCg--- -3' miRNA: 3'- ucUUUGUGGUUUUCGgGgGCGGacug -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 28381 | 0.68 | 0.556584 |
Target: 5'- ---cGCGCCAagGAAGCCgCgcugCGCCUGACc -3' miRNA: 3'- ucuuUGUGGU--UUUCGGgG----GCGGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 19644 | 0.67 | 0.636443 |
Target: 5'- cAGGAAUACCAGAAGCagaCCgaaGCCUa-- -3' miRNA: 3'- -UCUUUGUGGUUUUCGg--GGg--CGGAcug -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 15158 | 0.66 | 0.704827 |
Target: 5'- --cAGCGCC-AGGGCCUgcggCCGCCgGGCg -3' miRNA: 3'- ucuUUGUGGuUUUCGGG----GGCGGaCUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 11576 | 0.67 | 0.613488 |
Target: 5'- cGAAGCcauGCCGcccAGGGCCgCCGgCUGGCc -3' miRNA: 3'- uCUUUG---UGGU---UUUCGGgGGCgGACUG- -5' |
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26811 | 3' | -53.6 | NC_005808.1 | + | 17937 | 0.67 | 0.613488 |
Target: 5'- cGGggGCAC--GAAGCCCCCGUa---- -3' miRNA: 3'- -UCuuUGUGguUUUCGGGGGCGgacug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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