Results 1 - 20 of 71 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26814 | 3' | -68.1 | NC_005809.1 | + | 28243 | 0.66 | 0.175628 |
Target: 5'- -aGCaGGCCCGGCaguucagccaaGGCGCgCGCCa--- -3' miRNA: 3'- ccCG-CCGGGCCGg----------CCGCG-GCGGcauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 32913 | 0.66 | 0.175628 |
Target: 5'- -uGCGGCCaCGGCCuGCacgGCUGCUGUu- -3' miRNA: 3'- ccCGCCGG-GCCGGcCG---CGGCGGCAuc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 9210 | 0.66 | 0.175628 |
Target: 5'- cGGCGGCgCGGCgcacguucugCGGCGCgC-CCGgcGg -3' miRNA: 3'- cCCGCCGgGCCG----------GCCGCG-GcGGCauC- -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 27692 | 0.66 | 0.171245 |
Target: 5'- uGGaauacaGGCCgCGcGCCGGCagcggcucGCCGCCGg-- -3' miRNA: 3'- cCCg-----CCGG-GC-CGGCCG--------CGGCGGCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 40435 | 0.66 | 0.16696 |
Target: 5'- cGGCGGCgCGGuugaCCGGCuGCUGCaCGa-- -3' miRNA: 3'- cCCGCCGgGCC----GGCCG-CGGCG-GCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 22490 | 0.66 | 0.16696 |
Target: 5'- gGGGCgGGCUgGGCUGGUcauGCCcGCCa--- -3' miRNA: 3'- -CCCG-CCGGgCCGGCCG---CGG-CGGcauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 29088 | 0.66 | 0.16696 |
Target: 5'- cGGGCgccgaggucaGGCCCGuGCgGGacuuGCUGCCGg-- -3' miRNA: 3'- -CCCG----------CCGGGC-CGgCCg---CGGCGGCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 38994 | 0.66 | 0.162771 |
Target: 5'- uGGCGGCCgCGGaUCGGUGUaCGCCc--- -3' miRNA: 3'- cCCGCCGG-GCC-GGCCGCG-GCGGcauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 25630 | 0.66 | 0.162771 |
Target: 5'- cGGCGGCaCGcuGCUGGaCGgCGCCGUGc -3' miRNA: 3'- cCCGCCGgGC--CGGCC-GCgGCGGCAUc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 37056 | 0.66 | 0.161533 |
Target: 5'- uGGGCGG-CCGGCguguccuacgacuuUGGCaGCauCGCCGUAc -3' miRNA: 3'- -CCCGCCgGGCCG--------------GCCG-CG--GCGGCAUc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 30728 | 0.66 | 0.158677 |
Target: 5'- gGGGUucGCCCaGGCCaaccgcGGCGCgGCCGg-- -3' miRNA: 3'- -CCCGc-CGGG-CCGG------CCGCGgCGGCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 28672 | 0.66 | 0.158677 |
Target: 5'- cGGCGGCCUcGCCaGCGCgGCgCGc-- -3' miRNA: 3'- cCCGCCGGGcCGGcCGCGgCG-GCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 16067 | 0.66 | 0.158677 |
Target: 5'- -cGUGGCC--GCCGcGCGCggCGCCGUAGa -3' miRNA: 3'- ccCGCCGGgcCGGC-CGCG--GCGGCAUC- -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 25308 | 0.66 | 0.158677 |
Target: 5'- cGGcCGGCCCuGCCaccGGCGCucguCGCCGg-- -3' miRNA: 3'- cCC-GCCGGGcCGG---CCGCG----GCGGCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 33186 | 0.66 | 0.154676 |
Target: 5'- uGGGCGcCCuCGGCaCGGCcaCCGCUGgcGa -3' miRNA: 3'- -CCCGCcGG-GCCG-GCCGc-GGCGGCauC- -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 3746 | 0.66 | 0.154676 |
Target: 5'- uGGUGGgCaC-GUCGGUGCCGCCGcUGGa -3' miRNA: 3'- cCCGCCgG-GcCGGCCGCGGCGGC-AUC- -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 30985 | 0.66 | 0.154676 |
Target: 5'- cGGCGGCCa-GCCGGCgGCC-CUGg-- -3' miRNA: 3'- cCCGCCGGgcCGGCCG-CGGcGGCauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 13236 | 0.66 | 0.154676 |
Target: 5'- uGGUucgaGGaCuuGGCCGGCGaCCGCCa--- -3' miRNA: 3'- cCCG----CC-GggCCGGCCGC-GGCGGcauc -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 31877 | 0.67 | 0.146947 |
Target: 5'- --uCGGCCgaGGCCGugcGCGCUGCCGUcaAGa -3' miRNA: 3'- cccGCCGGg-CCGGC---CGCGGCGGCA--UC- -5' |
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26814 | 3' | -68.1 | NC_005809.1 | + | 23739 | 0.67 | 0.146947 |
Target: 5'- cGGUGGCgCGcagcagcgccauGCCGGCGCCaCCGg-- -3' miRNA: 3'- cCCGCCGgGC------------CGGCCGCGGcGGCauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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