Results 1 - 20 of 77 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26833 | 5' | -54.2 | NC_005809.1 | + | 17804 | 0.66 | 0.704773 |
Target: 5'- cGCGCGCAGuauUCcGCCuGCACgGa--- -3' miRNA: 3'- uCGCGCGUCu--AGuUGG-CGUGgCaaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 29507 | 0.66 | 0.704773 |
Target: 5'- gAGCGCcaauucaacauGCAGA-CGGCCGCACUcaaGCa -3' miRNA: 3'- -UCGCG-----------CGUCUaGUUGGCGUGGcaaUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 11586 | 0.66 | 0.704773 |
Target: 5'- cGCGCgugaGCAGAUCGuccACCGUcacGCCGa--- -3' miRNA: 3'- uCGCG----CGUCUAGU---UGGCG---UGGCaaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 4193 | 0.66 | 0.704773 |
Target: 5'- uGGCGgGCAuGUUGGCCGCGagcaCGUcGCg -3' miRNA: 3'- -UCGCgCGUcUAGUUGGCGUg---GCAaUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 28202 | 0.66 | 0.693593 |
Target: 5'- cGGCGCcuugucCAGGUCGGCCaCGCCGa--- -3' miRNA: 3'- -UCGCGc-----GUCUAGUUGGcGUGGCaaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 3009 | 0.66 | 0.693593 |
Target: 5'- aGGCGCgGCGGGUCAuaGCUGC-UCGUc-- -3' miRNA: 3'- -UCGCG-CGUCUAGU--UGGCGuGGCAaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 25088 | 0.66 | 0.693593 |
Target: 5'- --aGCGCAGcgCGcCCGgCGCCGUgggaUACa -3' miRNA: 3'- ucgCGCGUCuaGUuGGC-GUGGCA----AUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 10872 | 0.66 | 0.693593 |
Target: 5'- cGGCGgGCuGGUCAggcgguGCUGCAgCaGUUACu -3' miRNA: 3'- -UCGCgCGuCUAGU------UGGCGUgG-CAAUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 18414 | 0.66 | 0.682349 |
Target: 5'- aAGCGCGCAGccGUCGaggcGCUGgGCCaGgcGCg -3' miRNA: 3'- -UCGCGCGUC--UAGU----UGGCgUGG-CaaUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 7634 | 0.66 | 0.682349 |
Target: 5'- gGGgGCGCGGcUCGACCcaCGCCGg--- -3' miRNA: 3'- -UCgCGCGUCuAGUUGGc-GUGGCaaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 7749 | 0.66 | 0.682349 |
Target: 5'- -cUGCGCAG-UCAGCgauugcaGCACCGgcGCg -3' miRNA: 3'- ucGCGCGUCuAGUUGg------CGUGGCaaUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 304 | 0.66 | 0.682349 |
Target: 5'- aGGCGCGCucuuccguccAGggCGuGCCGUACCGggGg -3' miRNA: 3'- -UCGCGCG----------UCuaGU-UGGCGUGGCaaUg -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 34738 | 0.66 | 0.681222 |
Target: 5'- cGUGCGCcuGGUCAucgugcaGCCGCGCCu---- -3' miRNA: 3'- uCGCGCGu-CUAGU-------UGGCGUGGcaaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 18467 | 0.66 | 0.681222 |
Target: 5'- cGGCGCcgGCGGcGUCGGCCGauucgucCACCGUgaugACc -3' miRNA: 3'- -UCGCG--CGUC-UAGUUGGC-------GUGGCAa---UG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 4531 | 0.66 | 0.671054 |
Target: 5'- uGGCGUGCuGggCggUCGUGCCGccGCg -3' miRNA: 3'- -UCGCGCGuCuaGuuGGCGUGGCaaUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 10597 | 0.66 | 0.659721 |
Target: 5'- aGGCGCGCAGc----CUGCACCGc--- -3' miRNA: 3'- -UCGCGCGUCuaguuGGCGUGGCaaug -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 19968 | 0.66 | 0.656315 |
Target: 5'- cGCGCGCGGggCGcuucuucgugcaagAUCGCaACCGcgGCg -3' miRNA: 3'- uCGCGCGUCuaGU--------------UGGCG-UGGCaaUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 7486 | 0.66 | 0.648359 |
Target: 5'- -uCGaC-CAGAUCGACCGCGCCcagcagGUUGCc -3' miRNA: 3'- ucGC-GcGUCUAGUUGGCGUGG------CAAUG- -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 11142 | 0.66 | 0.648359 |
Target: 5'- cGC-CGCAGAUCGuggCGuCGCCGUUGa -3' miRNA: 3'- uCGcGCGUCUAGUug-GC-GUGGCAAUg -5' |
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26833 | 5' | -54.2 | NC_005809.1 | + | 13816 | 0.66 | 0.648359 |
Target: 5'- uGCGCGCcGAgaagCGcACCGCGCUGg--- -3' miRNA: 3'- uCGCGCGuCUa---GU-UGGCGUGGCaaug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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