Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27024 | 3' | -55.9 | NC_005832.1 | + | 80112 | 0.66 | 0.842899 |
Target: 5'- gGGACguugggGUCCcccaccuCCCUCCUCCACc -3' miRNA: 3'- -UCUGaga---UAGGacgu---GGGAGGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 13431 | 0.66 | 0.842899 |
Target: 5'- aAGACcaUCg--CCguggacgGCACCUUCCUCCuGCa -3' miRNA: 3'- -UCUG--AGauaGGa------CGUGGGAGGAGG-UG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 8036 | 0.66 | 0.825747 |
Target: 5'- ---gUCUGUCCU-CACCCUCUcCCAg -3' miRNA: 3'- ucugAGAUAGGAcGUGGGAGGaGGUg -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 85560 | 0.66 | 0.816884 |
Target: 5'- uGAC-CUGcucUCCUGUAgCCUCgUCCAg -3' miRNA: 3'- uCUGaGAU---AGGACGUgGGAGgAGGUg -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 28235 | 0.66 | 0.807841 |
Target: 5'- gGGACUUguagUCUcCGCCCgcaaCCUCCACg -3' miRNA: 3'- -UCUGAGaua-GGAcGUGGGa---GGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 320 | 0.67 | 0.789252 |
Target: 5'- uGACUUU-UCCaaGUAcguCCCUCCUCCGCc -3' miRNA: 3'- uCUGAGAuAGGa-CGU---GGGAGGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 1296 | 0.67 | 0.789252 |
Target: 5'- uGGugUCUGagacUCCaGCGucCCCUCCccagCCACa -3' miRNA: 3'- -UCugAGAU----AGGaCGU--GGGAGGa---GGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 78595 | 0.67 | 0.78641 |
Target: 5'- uGGACUCUGcggcgcccaccggcUCgUGC-CCCUacaUCUCCGCu -3' miRNA: 3'- -UCUGAGAU--------------AGgACGuGGGA---GGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 10461 | 0.67 | 0.779725 |
Target: 5'- uAGuCUC---CCUGCcggugGCUCUCCUCCACc -3' miRNA: 3'- -UCuGAGauaGGACG-----UGGGAGGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 83339 | 0.67 | 0.779725 |
Target: 5'- -aACUCUGUUCUGUACUCgacgUCgUCCAUg -3' miRNA: 3'- ucUGAGAUAGGACGUGGG----AGgAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 54101 | 0.67 | 0.770057 |
Target: 5'- cGGACUCUAgggucuuuacaUCCgGCGCuCCcCCUCC-Cg -3' miRNA: 3'- -UCUGAGAU-----------AGGaCGUG-GGaGGAGGuG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 32292 | 0.67 | 0.760257 |
Target: 5'- uGGACUCc--CCU-CGCCgCUCCUCCGg -3' miRNA: 3'- -UCUGAGauaGGAcGUGG-GAGGAGGUg -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 20991 | 0.67 | 0.750335 |
Target: 5'- uGGCUCUGUCCUccaggaucccaGCuCCCgccggCCUCCu- -3' miRNA: 3'- uCUGAGAUAGGA-----------CGuGGGa----GGAGGug -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 39415 | 0.67 | 0.740301 |
Target: 5'- uGGACUCcg-UCUGCGCCCgcggggccCCUUUACg -3' miRNA: 3'- -UCUGAGauaGGACGUGGGa-------GGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 66765 | 0.68 | 0.709635 |
Target: 5'- cGGACgccacguUCCUGC-CCCUCgUCCAg -3' miRNA: 3'- -UCUGagau---AGGACGuGGGAGgAGGUg -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 63046 | 0.69 | 0.657246 |
Target: 5'- uAGAUUCUAucuUCCUGggggccaaACCCUCCaUCCAg -3' miRNA: 3'- -UCUGAGAU---AGGACg-------UGGGAGG-AGGUg -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 70580 | 0.69 | 0.646665 |
Target: 5'- uAGGCUCUcuuAUCCUGCGCUUUCU--CACa -3' miRNA: 3'- -UCUGAGA---UAGGACGUGGGAGGagGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 44979 | 0.69 | 0.64031 |
Target: 5'- gGGACUCUgagaccccgugagagAUUUUGCACCaCUgCUCCAUc -3' miRNA: 3'- -UCUGAGA---------------UAGGACGUGG-GAgGAGGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 100615 | 0.69 | 0.636072 |
Target: 5'- uGGACUCUAcUCCgGC-CUCUCCcauagCCGCa -3' miRNA: 3'- -UCUGAGAU-AGGaCGuGGGAGGa----GGUG- -5' |
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27024 | 3' | -55.9 | NC_005832.1 | + | 26937 | 0.69 | 0.625476 |
Target: 5'- uGcCUCUGcaagCgUGCACCCUCCUgcucCCGCa -3' miRNA: 3'- uCuGAGAUa---GgACGUGGGAGGA----GGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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