Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27026 | 3' | -59.7 | NC_005832.1 | + | 37948 | 0.65 | 0.714387 |
Target: 5'- aGGCGugcCAGGGCCGUaccccauCGCCacCGUGGGAg -3' miRNA: 3'- cCUGU---GUCCUGGCG-------GCGGc-GCAUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 65581 | 0.66 | 0.705495 |
Target: 5'- ---gGCAGGACCcugcCCGCCGgG-GGGAg -3' miRNA: 3'- ccugUGUCCUGGc---GGCGGCgCaUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 14516 | 0.66 | 0.698545 |
Target: 5'- cGACGCAuacugcgagccguguGGACUGCgcaacaaUGUCGCGUAGGc -3' miRNA: 3'- cCUGUGU---------------CCUGGCG-------GCGGCGCAUCCu -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 60160 | 0.66 | 0.698545 |
Target: 5'- cGGACguagcuccccucaACAGGucuaugugguuccugACUGCCGCCGCGauaccccauGGAg -3' miRNA: 3'- -CCUG-------------UGUCC---------------UGGCGGCGGCGCau-------CCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 1272 | 0.66 | 0.685568 |
Target: 5'- uGGAUAUAGaGGCCGCCGaCCuguccuCGcGGGAc -3' miRNA: 3'- -CCUGUGUC-CUGGCGGC-GGc-----GCaUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 66122 | 0.66 | 0.675535 |
Target: 5'- cGACGCAGagugugaaaaGAUUGCCGCgGCGggaauGGAg -3' miRNA: 3'- cCUGUGUC----------CUGGCGGCGgCGCau---CCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 35746 | 0.66 | 0.675535 |
Target: 5'- aGACG-GGGGCUGCUGCUGCuUGGGu -3' miRNA: 3'- cCUGUgUCCUGGCGGCGGCGcAUCCu -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 47403 | 0.67 | 0.655374 |
Target: 5'- cGGugACGGGAUCcuuGuuGCUGCccAGGAg -3' miRNA: 3'- -CCugUGUCCUGG---CggCGGCGcaUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 98713 | 0.68 | 0.594702 |
Target: 5'- aGGCACAGGGCCGUgaaCGCCGgccCGUAc-- -3' miRNA: 3'- cCUGUGUCCUGGCG---GCGGC---GCAUccu -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 10517 | 0.68 | 0.593695 |
Target: 5'- aGGgACAGG-CCGCUGCCGUugacguacucgcaGUAGGc -3' miRNA: 3'- cCUgUGUCCuGGCGGCGGCG-------------CAUCCu -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 24731 | 0.68 | 0.56463 |
Target: 5'- aGACcaAGGGCCGcCCGCUGCucAGGAg -3' miRNA: 3'- cCUGugUCCUGGC-GGCGGCGcaUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 97112 | 0.68 | 0.544801 |
Target: 5'- uGGA-GCAGG-CCGCCGCgGUGgcccugAGGGc -3' miRNA: 3'- -CCUgUGUCCuGGCGGCGgCGCa-----UCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 55991 | 0.69 | 0.534971 |
Target: 5'- aGACACGGGGCCcgcguaGCCGUaCGCGgcccuGGGu -3' miRNA: 3'- cCUGUGUCCUGG------CGGCG-GCGCau---CCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 95169 | 0.69 | 0.525204 |
Target: 5'- -aGCGCAGGuacaaacCCaCCGUCGUGUGGGAc -3' miRNA: 3'- ccUGUGUCCu------GGcGGCGGCGCAUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 73025 | 0.69 | 0.515505 |
Target: 5'- cGGACcguCAGGACCcuuugCGCC-CGUGGGAc -3' miRNA: 3'- -CCUGu--GUCCUGGcg---GCGGcGCAUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 662 | 0.69 | 0.50588 |
Target: 5'- cGGACGC-GGACC-CCGCCGgGggcuGGu -3' miRNA: 3'- -CCUGUGuCCUGGcGGCGGCgCau--CCu -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 72061 | 0.69 | 0.486867 |
Target: 5'- cGugAUAGuGGCgGCCGUCGUgGUGGGAg -3' miRNA: 3'- cCugUGUC-CUGgCGGCGGCG-CAUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 97163 | 0.69 | 0.486867 |
Target: 5'- gGGAgGcCAGGuCCGCCaCCaCGUAGGAc -3' miRNA: 3'- -CCUgU-GUCCuGGCGGcGGcGCAUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 36020 | 0.69 | 0.486867 |
Target: 5'- cGGGCccuCAGGGCCagagcuCCuaCGCGUGGGAu -3' miRNA: 3'- -CCUGu--GUCCUGGc-----GGcgGCGCAUCCU- -5' |
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27026 | 3' | -59.7 | NC_005832.1 | + | 95554 | 0.7 | 0.456263 |
Target: 5'- uGGACACGGGcuagccuccgcgGCCGCUGCCG-GUGacugucucauugccGGAg -3' miRNA: 3'- -CCUGUGUCC------------UGGCGGCGGCgCAU--------------CCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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