Results 1 - 20 of 67 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27028 | 5' | -56.5 | NC_005832.1 | + | 43351 | 0.66 | 0.859242 |
Target: 5'- -aGAC-GC-GGACCCcgCAGCGCCGg- -3' miRNA: 3'- ggCUGcCGuUCUGGGa-GUUGCGGCag -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 78577 | 0.66 | 0.851388 |
Target: 5'- -gGACGGCGuacgagauuguGGACUCUgCGGCGCCc-- -3' miRNA: 3'- ggCUGCCGU-----------UCUGGGA-GUUGCGGcag -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 9619 | 0.66 | 0.851388 |
Target: 5'- cCUGG-GGCAGGcuguCCCUCAG-GUCGUCu -3' miRNA: 3'- -GGCUgCCGUUCu---GGGAGUUgCGGCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 86521 | 0.66 | 0.851388 |
Target: 5'- gCGGCGGcCAAGaacgaaGCCUUgGGCGCCuUCu -3' miRNA: 3'- gGCUGCC-GUUC------UGGGAgUUGCGGcAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 74455 | 0.66 | 0.843332 |
Target: 5'- cCUGACGGg-----CCUgGACGCCGUCa -3' miRNA: 3'- -GGCUGCCguucugGGAgUUGCGGCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 9102 | 0.66 | 0.843332 |
Target: 5'- gCGugGGguauaAAGuCCCUCc-UGCCGUCa -3' miRNA: 3'- gGCugCCg----UUCuGGGAGuuGCGGCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 39410 | 0.66 | 0.843332 |
Target: 5'- uCCGucuGCGcccGCGGGGcCCCUUuACGCCGUUc -3' miRNA: 3'- -GGC---UGC---CGUUCU-GGGAGuUGCGGCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 72796 | 0.66 | 0.843332 |
Target: 5'- -gGACcaaCGGGACCCUUuGgGCCGUCg -3' miRNA: 3'- ggCUGcc-GUUCUGGGAGuUgCGGCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 12011 | 0.66 | 0.835081 |
Target: 5'- -aGACGGa---GCCCUCAcCGCCGa- -3' miRNA: 3'- ggCUGCCguucUGGGAGUuGCGGCag -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 72962 | 0.66 | 0.826642 |
Target: 5'- cCCGACGGacccCAGGGUCCUCGGgGCCc-- -3' miRNA: 3'- -GGCUGCC----GUUCUGGGAGUUgCGGcag -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 74751 | 0.66 | 0.826642 |
Target: 5'- cCCGcuauaACGGCGAgggaagcgguGACCCUCuuuaGCuCGUCg -3' miRNA: 3'- -GGC-----UGCCGUU----------CUGGGAGuug-CG-GCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 98717 | 0.66 | 0.826642 |
Target: 5'- -aGGCaGGCAcaGGGCCgUgAACGCCGg- -3' miRNA: 3'- ggCUG-CCGU--UCUGGgAgUUGCGGCag -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 2418 | 0.66 | 0.818023 |
Target: 5'- cCUGACGGCGuuggAGACggCCagCAACGCCa-- -3' miRNA: 3'- -GGCUGCCGU----UCUG--GGa-GUUGCGGcag -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 20494 | 0.66 | 0.818023 |
Target: 5'- -gGACGGU---GCCCUUGAUGCUGUa -3' miRNA: 3'- ggCUGCCGuucUGGGAGUUGCGGCAg -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 10191 | 0.67 | 0.809231 |
Target: 5'- -aGGCGGUggagAGGAUCCUCAugacuucCGCCGcCg -3' miRNA: 3'- ggCUGCCG----UUCUGGGAGUu------GCGGCaG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 12068 | 0.67 | 0.809231 |
Target: 5'- gCCGGCGGCAaacgcagaaGGAUCggUCAcguAgGCCGUCu -3' miRNA: 3'- -GGCUGCCGU---------UCUGGg-AGU---UgCGGCAG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 54480 | 0.67 | 0.809231 |
Target: 5'- cCCGcCGaaaAAGGCCCcCAGCGCCG-Cg -3' miRNA: 3'- -GGCuGCcg-UUCUGGGaGUUGCGGCaG- -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 90909 | 0.67 | 0.803877 |
Target: 5'- gCCGAgGGCGGGcCCCUCaggaggcuccuuacgAggcagcuggACGCCGUg -3' miRNA: 3'- -GGCUgCCGUUCuGGGAG---------------U---------UGCGGCAg -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 32316 | 0.67 | 0.800276 |
Target: 5'- uUGugGGCuccGGACCC-CA-UGCCGUg -3' miRNA: 3'- gGCugCCGu--UCUGGGaGUuGCGGCAg -5' |
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27028 | 5' | -56.5 | NC_005832.1 | + | 29243 | 0.67 | 0.800276 |
Target: 5'- ----aGGCucacGACCgagCUCAACGCCGUCc -3' miRNA: 3'- ggcugCCGuu--CUGG---GAGUUGCGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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