Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27032 | 3' | -63.2 | NC_005832.1 | + | 96210 | 0.66 | 0.520755 |
Target: 5'- cGuuGAGGaCCaGUCCGGuGACGGCCCaCGa -3' miRNA: 3'- -CucCUCCcGG-CAGGUC-CUGCUGGG-GC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 103474 | 0.66 | 0.520755 |
Target: 5'- -cGGAGGuCCucgggGUCCAGGACuacgcaacuGCCCCGg -3' miRNA: 3'- cuCCUCCcGG-----CAGGUCCUGc--------UGGGGC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 65717 | 0.66 | 0.517904 |
Target: 5'- aGAGGcugAGGGCCcuccagauauccauGUCCAGGccCGACCUgGc -3' miRNA: 3'- -CUCC---UCCCGG--------------CAGGUCCu-GCUGGGgC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 20592 | 0.66 | 0.511276 |
Target: 5'- cGGGAGGGuuGcCCAucaugcGGACaGACaCCCa -3' miRNA: 3'- cUCCUCCCggCaGGU------CCUG-CUG-GGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 3113 | 0.66 | 0.511276 |
Target: 5'- uGGGuuGGuGCCGUCCAGGuCGuACUCUa -3' miRNA: 3'- cUCCu-CC-CGGCAGGUCCuGC-UGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 78195 | 0.66 | 0.508447 |
Target: 5'- aAGGuguuuaaucugcuaGGGGCCGUagagggaaAGGACGACCUCc -3' miRNA: 3'- cUCC--------------UCCCGGCAgg------UCCUGCUGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 52703 | 0.66 | 0.501871 |
Target: 5'- gGAGuGAGGGCCGUgguGGugGACUUgGg -3' miRNA: 3'- -CUC-CUCCCGGCAgguCCugCUGGGgC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 72819 | 0.66 | 0.501871 |
Target: 5'- cGGGAGGGCC-UCCuaaaccccuGGGACcaacggGACCCUu -3' miRNA: 3'- cUCCUCCCGGcAGG---------UCCUG------CUGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 54372 | 0.66 | 0.492541 |
Target: 5'- cGGGGAGGacaGCaCGguggaCAGGGuCGGCCCCa -3' miRNA: 3'- -CUCCUCC---CG-GCag---GUCCU-GCUGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 613 | 0.66 | 0.483293 |
Target: 5'- cAGGGGccCCGaCCAGGACGccGCCCUGg -3' miRNA: 3'- cUCCUCccGGCaGGUCCUGC--UGGGGC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 96685 | 0.66 | 0.482372 |
Target: 5'- cGAGGAGGccggcaaGCUGUCCGaGAUGGCCgCa -3' miRNA: 3'- -CUCCUCC-------CGGCAGGUcCUGCUGGgGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 17106 | 0.67 | 0.474128 |
Target: 5'- --uGAGGGCCaUCCAGGACcuccuGAUCCUc -3' miRNA: 3'- cucCUCCCGGcAGGUCCUG-----CUGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 1385 | 0.67 | 0.429689 |
Target: 5'- uAGGAGGGacaggaggaUGcCCAGGAUGuuCCCCGu -3' miRNA: 3'- cUCCUCCCg--------GCaGGUCCUGCu-GGGGC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 41564 | 0.68 | 0.421097 |
Target: 5'- gGAGGAGGccgccGCCGUCaGGGAgGcGCCCuCGg -3' miRNA: 3'- -CUCCUCC-----CGGCAGgUCCUgC-UGGG-GC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 90009 | 0.68 | 0.41261 |
Target: 5'- uGAGGuGGGCCGUgauGGAgGAgCCCa -3' miRNA: 3'- -CUCCuCCCGGCAgguCCUgCUgGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 96769 | 0.68 | 0.41261 |
Target: 5'- gGAGGGGGuGacaaCGUCCAcGGCGuCCCUGg -3' miRNA: 3'- -CUCCUCC-Cg---GCAGGUcCUGCuGGGGC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 101880 | 0.68 | 0.404229 |
Target: 5'- --uGAGGGCCGggaAGGGCuugGACCCCa -3' miRNA: 3'- cucCUCCCGGCaggUCCUG---CUGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 72376 | 0.68 | 0.395957 |
Target: 5'- aGGGAGGGCCaacggaaCCaaAGGGCGAcaagggacCCCCGg -3' miRNA: 3'- cUCCUCCCGGca-----GG--UCCUGCU--------GGGGC- -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 32036 | 0.68 | 0.394316 |
Target: 5'- aGGGAGGgucaGCCGUCCugAGGGagcccaaucccaGACCCCa -3' miRNA: 3'- cUCCUCC----CGGCAGG--UCCUg-----------CUGGGGc -5' |
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27032 | 3' | -63.2 | NC_005832.1 | + | 80104 | 0.68 | 0.387795 |
Target: 5'- aGGGGGaGGCUcagguacagGUCCAGGA-GACCCaCGg -3' miRNA: 3'- cUCCUC-CCGG---------CAGGUCCUgCUGGG-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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