Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27033 | 3' | -53.2 | NC_005832.1 | + | 44308 | 0.71 | 0.708603 |
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Target: 5'- --cUGACGGCaaa-AAGCUCGUCAAGg -3' miRNA: 3'- gucACUGCCGggagUUCGAGUAGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 93703 | 0.71 | 0.666046 |
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Target: 5'- gGGaGGCGGCCUgaccguaaaCAAGCUCAUCAGc -3' miRNA: 3'- gUCaCUGCCGGGa--------GUUCGAGUAGUUc -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 102883 | 0.72 | 0.622998 |
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Target: 5'- -uGUGGCGGCCCUCAucuacaugGGCUa--CAGGa -3' miRNA: 3'- guCACUGCCGGGAGU--------UCGAguaGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 79168 | 0.73 | 0.580068 |
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Target: 5'- --uUGugGGCCCUguUGAGCUCGUCGGu -3' miRNA: 3'- gucACugCCGGGA--GUUCGAGUAGUUc -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 87375 | 0.73 | 0.569419 |
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Target: 5'- --uUGACauGGCCCUCAGGCUCucCAGGg -3' miRNA: 3'- gucACUG--CCGGGAGUUCGAGuaGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 3935 | 0.79 | 0.274242 |
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Target: 5'- uGG-GAUGGCCCUCAAGCUCGUgAu- -3' miRNA: 3'- gUCaCUGCCGGGAGUUCGAGUAgUuc -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 12717 | 1.1 | 0.002867 |
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Target: 5'- cCAGUGACGGCCCUCAAGCUCAUCAAGg -3' miRNA: 3'- -GUCACUGCCGGGAGUUCGAGUAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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