Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27033 | 3' | -53.2 | NC_005832.1 | + | 93703 | 0.71 | 0.666046 |
|
Target: 5'- gGGaGGCGGCCUgaccguaaaCAAGCUCAUCAGc -3' miRNA: 3'- gUCaCUGCCGGGa--------GUUCGAGUAGUUc -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 94067 | 0.68 | 0.860566 |
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Target: 5'- -cGUGGCGGCCCUgAuguacuugaGGCccgaacCGUCGAGg -3' miRNA: 3'- guCACUGCCGGGAgU---------UCGa-----GUAGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 94207 | 0.7 | 0.750008 |
|
Target: 5'- aCGGUu-CGGgCCUCAAGUaCAUCAGGg -3' miRNA: 3'- -GUCAcuGCCgGGAGUUCGaGUAGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 95774 | 0.67 | 0.890851 |
|
Target: 5'- cUAGUGGCGGCuacCCUCGGGCUgGc---- -3' miRNA: 3'- -GUCACUGCCG---GGAGUUCGAgUaguuc -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 96786 | 0.66 | 0.92868 |
|
Target: 5'- aGGUGgACGGCCUcaagcagacaugUCGGGCU--UCAAGa -3' miRNA: 3'- gUCAC-UGCCGGG------------AGUUCGAguAGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 102883 | 0.72 | 0.622998 |
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Target: 5'- -uGUGGCGGCCCUCAucuacaugGGCUa--CAGGa -3' miRNA: 3'- guCACUGCCGGGAGU--------UCGAguaGUUC- -5' |
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| 27033 | 3' | -53.2 | NC_005832.1 | + | 104099 | 0.69 | 0.779895 |
|
Target: 5'- ---cGGCGGCCCUCAGGgccaccauCUCcUCGGGa -3' miRNA: 3'- gucaCUGCCGGGAGUUC--------GAGuAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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