Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27034 | 5' | -57.5 | NC_005832.1 | + | 94195 | 0.66 | 0.721102 |
Target: 5'- gAAAG-CCGggcuGGGAGGCCCUCuuuccGCCa-- -3' miRNA: 3'- -UUUCaGGU----UCCUCCGGGAGc----CGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 38715 | 0.66 | 0.721102 |
Target: 5'- cGGAGcUCGAGGAGGCUUUCguagaccuGGCCUUc -3' miRNA: 3'- -UUUCaGGUUCCUCCGGGAG--------CCGGAAu -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 96365 | 0.66 | 0.721102 |
Target: 5'- ---cUCCGAGGAGagcucagcGCCCugUCGGCCg-- -3' miRNA: 3'- uuucAGGUUCCUC--------CGGG--AGCCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 28064 | 0.66 | 0.707652 |
Target: 5'- --uGUCCAGGGugguggggaccaagAGGCCCUUuGCCg-- -3' miRNA: 3'- uuuCAGGUUCC--------------UCCGGGAGcCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 78277 | 0.66 | 0.70036 |
Target: 5'- --cGUCCuuGaAGGUCCUUGGCCUg- -3' miRNA: 3'- uuuCAGGuuCcUCCGGGAGCCGGAau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 12992 | 0.66 | 0.70036 |
Target: 5'- cGGGGUCCGucAGGcugggguccAGGCCCcUGGUCUUGa -3' miRNA: 3'- -UUUCAGGU--UCC---------UCCGGGaGCCGGAAU- -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 54943 | 0.66 | 0.689892 |
Target: 5'- cGGGGUCC-AGGAcGGUCCccccgUCGGCCcUUAg -3' miRNA: 3'- -UUUCAGGuUCCU-CCGGG-----AGCCGG-AAU- -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 66843 | 0.67 | 0.662455 |
Target: 5'- --uGUCCAGGGAcagguccccaaacacGGUCC-CGGCCg-- -3' miRNA: 3'- uuuCAGGUUCCU---------------CCGGGaGCCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 48296 | 0.67 | 0.658214 |
Target: 5'- uAAAGUCCugcaGGGCCCUggucaCGGCCUg- -3' miRNA: 3'- -UUUCAGGuuccUCCGGGA-----GCCGGAau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 43130 | 0.67 | 0.647597 |
Target: 5'- uGGAGacacCCAGGGAGGCCgUgcUGGCCa-- -3' miRNA: 3'- -UUUCa---GGUUCCUCCGGgA--GCCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 49006 | 0.67 | 0.647597 |
Target: 5'- uAAAGUCCcugacguaGAGGAGGUCgCacucgaCGGCCUUGg -3' miRNA: 3'- -UUUCAGG--------UUCCUCCGG-Ga-----GCCGGAAU- -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 2137 | 0.68 | 0.605085 |
Target: 5'- uAAAGUCCGAGGGGGa-CagGGCCa-- -3' miRNA: 3'- -UUUCAGGUUCCUCCggGagCCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 79289 | 0.68 | 0.594491 |
Target: 5'- ----gCCAGGGAGGCCgCUCcacccaGGCCUc- -3' miRNA: 3'- uuucaGGUUCCUCCGG-GAG------CCGGAau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 43240 | 0.68 | 0.583926 |
Target: 5'- ---cUCCAGGGAGaaccccaccCCCUCGGCCa-- -3' miRNA: 3'- uuucAGGUUCCUCc--------GGGAGCCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 92557 | 0.68 | 0.573398 |
Target: 5'- cGGGUCCGAGGAcgcgacggggacGGCUCUgGGCUg-- -3' miRNA: 3'- uUUCAGGUUCCU------------CCGGGAgCCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 104105 | 0.68 | 0.573398 |
Target: 5'- --cGUCCGcGGcGGCCCUCagGGCCa-- -3' miRNA: 3'- uuuCAGGUuCCuCCGGGAG--CCGGaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 49515 | 0.68 | 0.562916 |
Target: 5'- aAGGGUCCGAGGAGGUCa--GGCg--- -3' miRNA: 3'- -UUUCAGGUUCCUCCGGgagCCGgaau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 28002 | 0.69 | 0.501342 |
Target: 5'- --cGcCUAAGGAGGUCCagGGCCUg- -3' miRNA: 3'- uuuCaGGUUCCUCCGGGagCCGGAau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 16980 | 0.7 | 0.481463 |
Target: 5'- gGAGGUCCu-GGAuGGCCCUCaGCUUg- -3' miRNA: 3'- -UUUCAGGuuCCU-CCGGGAGcCGGAau -5' |
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27034 | 5' | -57.5 | NC_005832.1 | + | 90776 | 0.7 | 0.461974 |
Target: 5'- aGGAGccUCCuGAGGGGcccGCCCUCGGCCg-- -3' miRNA: 3'- -UUUC--AGG-UUCCUC---CGGGAGCCGGaau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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