Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27034 | 5' | -57.5 | NC_005832.1 | + | 90387 | 0.7 | 0.442909 |
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Target: 5'- cAAAGUCCAcGGGGGCCCUCcucgacaGCUUg- -3' miRNA: 3'- -UUUCAGGUuCCUCCGGGAGc------CGGAau -5' |
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| 27034 | 5' | -57.5 | NC_005832.1 | + | 89565 | 0.71 | 0.397274 |
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Target: 5'- cAGG-CCAGGGGGGCCgUCaGCCUg- -3' miRNA: 3'- uUUCaGGUUCCUCCGGgAGcCGGAau -5' |
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| 27034 | 5' | -57.5 | NC_005832.1 | + | 100222 | 0.72 | 0.346667 |
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Target: 5'- cGGGGUCCGAGGAGGCaaaCGGCUUc- -3' miRNA: 3'- -UUUCAGGUUCCUCCGggaGCCGGAau -5' |
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| 27034 | 5' | -57.5 | NC_005832.1 | + | 80021 | 0.74 | 0.279747 |
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Target: 5'- aAGGGUCCGAGGccauGGCcgcccucuCCUCGGCCUg- -3' miRNA: 3'- -UUUCAGGUUCCu---CCG--------GGAGCCGGAau -5' |
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| 27034 | 5' | -57.5 | NC_005832.1 | + | 53913 | 0.75 | 0.253469 |
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Target: 5'- aGGGGUCCugAAGGAGGCCUggGGCCa-- -3' miRNA: 3'- -UUUCAGG--UUCCUCCGGGagCCGGaau -5' |
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| 27034 | 5' | -57.5 | NC_005832.1 | + | 51887 | 0.78 | 0.14223 |
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Target: 5'- uAAAGaCCAGGGAGGCCCUggguauggcucaggCGGCCUUu -3' miRNA: 3'- -UUUCaGGUUCCUCCGGGA--------------GCCGGAAu -5' |
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| 27034 | 5' | -57.5 | NC_005832.1 | + | 12815 | 1.04 | 0.002143 |
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Target: 5'- uAAAGUCCAAGGAGGCCCUCGGCCUUAa -3' miRNA: 3'- -UUUCAGGUUCCUCCGGGAGCCGGAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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