Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 27035 | 3' | -57.2 | NC_005832.1 | + | 11780 | 0.66 | 0.777982 |
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Target: 5'- -uGGCUCGgccggGGGGAGGUCgacgagggcacccugCUGGACGc -3' miRNA: 3'- gcUCGAGUa----CUCCUCCAGg--------------GACCUGC- -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 59899 | 0.66 | 0.786389 |
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Target: 5'- uGAGaUCcuggAUGAGGAGG-CUCUGGAgGg -3' miRNA: 3'- gCUCgAG----UACUCCUCCaGGGACCUgC- -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 66706 | 0.66 | 0.795595 |
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Target: 5'- cCGGGaC-CGUGuuuGGGGaccuGUCCCUGGACa -3' miRNA: 3'- -GCUC-GaGUACu--CCUC----CAGGGACCUGc -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 32334 | 0.66 | 0.80465 |
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Target: 5'- gGGGCUg--GAGGGGGaUCCUugUGGGCu -3' miRNA: 3'- gCUCGAguaCUCCUCC-AGGG--ACCUGc -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 39241 | 0.66 | 0.80465 |
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Target: 5'- -aGGCUCGggaucGGGGAccgugaaccuggGGUCCC-GGACGg -3' miRNA: 3'- gcUCGAGUa----CUCCU------------CCAGGGaCCUGC- -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 11353 | 0.66 | 0.812663 |
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Target: 5'- aCGAGCUCGuguaaaaaguuucUGAGGAGcGccgcucCCCUGGcCa -3' miRNA: 3'- -GCUCGAGU-------------ACUCCUC-Ca-----GGGACCuGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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