Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 27035 | 3' | -57.2 | NC_005832.1 | + | 66706 | 0.66 | 0.795595 |
|
Target: 5'- cCGGGaC-CGUGuuuGGGGaccuGUCCCUGGACa -3' miRNA: 3'- -GCUC-GaGUACu--CCUC----CAGGGACCUGc -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 72567 | 0.68 | 0.69726 |
|
Target: 5'- uGGGgaCGUGGGGccccugggcuucaGGGUCCCgacgucguagUGGACGg -3' miRNA: 3'- gCUCgaGUACUCC-------------UCCAGGG----------ACCUGC- -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 75757 | 0.68 | 0.702342 |
|
Target: 5'- aGGGC-CGUGAGGuccgcugcgggcgagAGGUCCauugguCUGGGCGc -3' miRNA: 3'- gCUCGaGUACUCC---------------UCCAGG------GACCUGC- -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 79116 | 0.71 | 0.514248 |
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Target: 5'- --cGCUUAcGAGGAGGUCCgUgaGGACGu -3' miRNA: 3'- gcuCGAGUaCUCCUCCAGGgA--CCUGC- -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 92603 | 0.67 | 0.745303 |
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Target: 5'- aGAGaaaaguacaaacucCUCAccAGGAGGcugUCCCUGGACa -3' miRNA: 3'- gCUC--------------GAGUacUCCUCC---AGGGACCUGc -5' |
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| 27035 | 3' | -57.2 | NC_005832.1 | + | 105021 | 0.69 | 0.626192 |
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Target: 5'- aGAcGC-CAUGAGGAGGUaCCaGGGCa -3' miRNA: 3'- gCU-CGaGUACUCCUCCAgGGaCCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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