Results 1 - 20 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27301 | 3' | -50.6 | NC_005856.1 | + | 72443 | 0.66 | 0.980649 |
Target: 5'- uCC-GCACGCU-GU-UCAAGCGAUCGa -3' miRNA: 3'- -GGuCGUGUGGuCGuAGUUCGUUAGCg -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 39235 | 0.66 | 0.980649 |
Target: 5'- aCCGGCuuACAUCAGC--UAAGCAaaagucAUUGCu -3' miRNA: 3'- -GGUCG--UGUGGUCGuaGUUCGU------UAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 25918 | 0.66 | 0.980649 |
Target: 5'- gCGGCGCAuUCGGCAUCAucauuGGCAGa--- -3' miRNA: 3'- gGUCGUGU-GGUCGUAGU-----UCGUUagcg -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 43909 | 0.66 | 0.978264 |
Target: 5'- aCCAGCGCAUC-GCGUUGguuuuGGCuuuuUUGCg -3' miRNA: 3'- -GGUCGUGUGGuCGUAGU-----UCGuu--AGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 73502 | 0.66 | 0.978264 |
Target: 5'- uCCAGUgucACACCuG-GUCG-GCAAUUGCg -3' miRNA: 3'- -GGUCG---UGUGGuCgUAGUuCGUUAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 28888 | 0.66 | 0.978014 |
Target: 5'- gUCAGCGCgcgagagugcccACCAGuCAUCcccgcagGGGCAaugguAUCGCg -3' miRNA: 3'- -GGUCGUG------------UGGUC-GUAG-------UUCGU-----UAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 91013 | 0.66 | 0.97647 |
Target: 5'- aUAGCGCGCgCGGCcuuccgcGcuucaacguuaucuaUCAGGUAAUCGCc -3' miRNA: 3'- gGUCGUGUG-GUCG-------U---------------AGUUCGUUAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 40426 | 0.66 | 0.972853 |
Target: 5'- gCCAGCgccGCAUCGGCAUacugaAAGCAccUGUa -3' miRNA: 3'- -GGUCG---UGUGGUCGUAg----UUCGUuaGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 56861 | 0.66 | 0.972853 |
Target: 5'- aCCGGUagauaacgucGCAUCGGCAugaUCGGGCuaacCGCg -3' miRNA: 3'- -GGUCG----------UGUGGUCGU---AGUUCGuua-GCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 2417 | 0.66 | 0.972853 |
Target: 5'- gCCGGUcguaccGCGCCGGguuGUCAuaaaaAGCAAUUGCc -3' miRNA: 3'- -GGUCG------UGUGGUCg--UAGU-----UCGUUAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 76527 | 0.66 | 0.972853 |
Target: 5'- --cGCACACCAGaAUCAcuaAGCAGgcCGUg -3' miRNA: 3'- gguCGUGUGGUCgUAGU---UCGUUa-GCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 58357 | 0.66 | 0.972853 |
Target: 5'- uCCAGCAuCGCUuuauuaguGGCAUCcauGCuAUUGCg -3' miRNA: 3'- -GGUCGU-GUGG--------UCGUAGuu-CGuUAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 14693 | 0.66 | 0.972853 |
Target: 5'- gUCGGCGCACCucGCuauUCuGGUAAUCa- -3' miRNA: 3'- -GGUCGUGUGGu-CGu--AGuUCGUUAGcg -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 29604 | 0.66 | 0.969809 |
Target: 5'- gUAGCACACCAGUggAUC-GGCGuGUCa- -3' miRNA: 3'- gGUCGUGUGGUCG--UAGuUCGU-UAGcg -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 78177 | 0.66 | 0.969809 |
Target: 5'- aCGGUAaGCCAGCAaaucCGGGCGAuaUUGCu -3' miRNA: 3'- gGUCGUgUGGUCGUa---GUUCGUU--AGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 58582 | 0.66 | 0.966187 |
Target: 5'- gCGGCACGCgCGGCAUCAucuuuaaugaauuGGUuAUCa- -3' miRNA: 3'- gGUCGUGUG-GUCGUAGU-------------UCGuUAGcg -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 85643 | 0.67 | 0.963005 |
Target: 5'- gCGGCGCugCGGCuUCAgaaAGCA--CGUa -3' miRNA: 3'- gGUCGUGugGUCGuAGU---UCGUuaGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 74475 | 0.67 | 0.963005 |
Target: 5'- gCCAGCGCGgUAGgAUCGGG--AUUGCu -3' miRNA: 3'- -GGUCGUGUgGUCgUAGUUCguUAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 49699 | 0.67 | 0.963005 |
Target: 5'- gCCAGCAaACUGGCAgguuUCuGAGCAgAUCGUc -3' miRNA: 3'- -GGUCGUgUGGUCGU----AG-UUCGU-UAGCG- -5' |
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27301 | 3' | -50.6 | NC_005856.1 | + | 13432 | 0.67 | 0.963005 |
Target: 5'- aCCAGCgaagACGacgaCAGUgaugauucaAUCAcgcAGCAAUCGCa -3' miRNA: 3'- -GGUCG----UGUg---GUCG---------UAGU---UCGUUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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