Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
27419 | 5' | -58.4 | NC_005869.1 | + | 33154 | 0.66 | 0.405534 |
Target: 5'- aGGGUGG-CGCaaGUgGGUccucaAACACCACa -3' miRNA: 3'- -UCCACCaGUGggCGgUCG-----UUGUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 17363 | 0.66 | 0.396128 |
Target: 5'- cGGcGGccgcCGCCCGCUc-CAGCGCCGCg -3' miRNA: 3'- uCCaCCa---GUGGGCGGucGUUGUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 12102 | 0.66 | 0.396128 |
Target: 5'- cAGGUGaUCGCCCagcGCgAGCGcucgaGCGCCAa -3' miRNA: 3'- -UCCACcAGUGGG---CGgUCGU-----UGUGGUg -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 24240 | 0.66 | 0.396128 |
Target: 5'- gGGGcaUGGccgaccgCAUCCGCCuGCAGCgcgccgGCCGCa -3' miRNA: 3'- -UCC--ACCa------GUGGGCGGuCGUUG------UGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 25988 | 0.66 | 0.386864 |
Target: 5'- aGGGUGGagcCcUCCGCCuGCGuCAUCACg -3' miRNA: 3'- -UCCACCa--GuGGGCGGuCGUuGUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 13650 | 0.66 | 0.386864 |
Target: 5'- gAGGaGGauga-UGCCAGCAGCGCCGCc -3' miRNA: 3'- -UCCaCCaguggGCGGUCGUUGUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 23420 | 0.66 | 0.37684 |
Target: 5'- cAGGg---CGCCgGCCGGCGGCuucaucuGCCGCa -3' miRNA: 3'- -UCCaccaGUGGgCGGUCGUUG-------UGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 24973 | 0.66 | 0.363456 |
Target: 5'- uGGUGGUCagcgacgagcagcugGCCCGCUGGaugcACACCuCg -3' miRNA: 3'- uCCACCAG---------------UGGGCGGUCgu--UGUGGuG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 17148 | 0.66 | 0.359068 |
Target: 5'- gGGGUGGgCACCgaGCCGGUGGugcagucCAUCACg -3' miRNA: 3'- -UCCACCaGUGGg-CGGUCGUU-------GUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 15059 | 0.67 | 0.333527 |
Target: 5'- gGGGUGGUgACCCcgaggguGCuCAGCAGCcgaACCu- -3' miRNA: 3'- -UCCACCAgUGGG-------CG-GUCGUUG---UGGug -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 30981 | 0.67 | 0.326129 |
Target: 5'- -uGUGuUCuccuCCCGCCGcuuguGCAGCACCACc -3' miRNA: 3'- ucCACcAGu---GGGCGGU-----CGUUGUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 24400 | 0.67 | 0.318052 |
Target: 5'- cAGGaGGcCGCCC-CCGGCGcaGCCGCg -3' miRNA: 3'- -UCCaCCaGUGGGcGGUCGUugUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 17883 | 0.68 | 0.272762 |
Target: 5'- gGGGUGG-CAuCCUGCCGGCGcuggugcccauCAUCGCg -3' miRNA: 3'- -UCCACCaGU-GGGCGGUCGUu----------GUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 13290 | 0.69 | 0.265737 |
Target: 5'- uAGGUguaGGUCGCCC-CCGGCAggaGCAgCAg -3' miRNA: 3'- -UCCA---CCAGUGGGcGGUCGU---UGUgGUg -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 15576 | 0.69 | 0.265737 |
Target: 5'- gAGGcGGagCACCCGgCGGCGGCGCagGCg -3' miRNA: 3'- -UCCaCCa-GUGGGCgGUCGUUGUGg-UG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 20079 | 0.69 | 0.252127 |
Target: 5'- cGGUGGUCgACCUGCaggaCAGgAAUACCGa -3' miRNA: 3'- uCCACCAG-UGGGCG----GUCgUUGUGGUg -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 26564 | 0.69 | 0.24554 |
Target: 5'- --cUGGUCACCCgcaccuucauGCCGcGCAACgGCCGCa -3' miRNA: 3'- uccACCAGUGGG----------CGGU-CGUUG-UGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 15345 | 0.69 | 0.24489 |
Target: 5'- -cGUGGUCAacgcgaUCGCCGGCGccacccgccgccgGCGCCGCc -3' miRNA: 3'- ucCACCAGUg-----GGCGGUCGU-------------UGUGGUG- -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 9902 | 0.69 | 0.239097 |
Target: 5'- cAGGUGGgugCGaccguagcCCCGCagggggcgCAGCAGCGCCAg -3' miRNA: 3'- -UCCACCa--GU--------GGGCG--------GUCGUUGUGGUg -5' |
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27419 | 5' | -58.4 | NC_005869.1 | + | 16640 | 0.69 | 0.239097 |
Target: 5'- gGGGUGGggggCACcaccgCCGUCAGCcGCACCu- -3' miRNA: 3'- -UCCACCa---GUG-----GGCGGUCGuUGUGGug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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